Reduction of SEM charging artefacts in native cryogenic biological samples.

Velazco, A.; Glen, T.; Klumpe, S.; Pennington, A.; Zhang, J.; Smith, J. L.; Glynn, C.; Bowles, W.; Kobylynska, M.; Fleck, R. A.; Naismith, J. H.; Kim, J. S.; Darrow, M. C.; Grange, M.; Kirkland, A. I.; Dumoux, M.

doi:10.1101/2024.08.23.609373

bioRxiv · 10.1101/2024.08.23.609373

Reduction of SEM charging artefacts in native cryogenic biological samples.

Velazco, A.·Glen, T.·Klumpe, S.·Pennington, A.·Zhang, J.·Smith, J. L.·Glynn, C.·Bowles, W.·Kobylynska, M.·Fleck, R. A.·Naismith, J. H.·Kim, J. S.·Darrow, M. C.·Grange, M.·Kirkland, A. I.·Dumoux, M.

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Abstract

Scanning electron microscopy (SEM) of frozen-hydrated biological samples allows imaging of subcellular structures at the mesoscale in their native state. Combined with focused ion beam milling (FIB), serial FIB/SEM can be used to build a 3-dimensional picture of cells and tissues. The correlation of specific regions of interest with cryo-electron microscopy (cryoEM) can additionally enable subsequent high-resolution analysis. However, the adoption of serial FIB/SEM imaging-based methods is limited due to artefacts arising from insulating areas of cryogenically preserved samples. Here, we demonstrate the use of interleaved scanning to reduce charging artefacts, allowing the observation of biological features that otherwise would be masked or perturbed. We apply our method to samples where inherent features are not visible. These examples include membrane contact sites within mammalian cells, visualisation of the degradation compartment in the algae E.gracilis and observation of a network of membranes within different types of axons in an adult mouse cortex. We further propose an alternative scanning method that could also be widely applicable to imaging any non-conductive.

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Velazco, A., Glen, T., Klumpe, S., Pennington, A., Zhang, J., Smith, J. L., Glynn, C., Bowles, W., Kobylynska, M., Fleck, R. A., Naismith, J. H., Kim, J. S., Darrow, M. C., Grange, M., Kirkland, A. I., Dumoux, M.. 2024-08-26. Reduction of SEM charging artefacts in native cryogenic biological samples.. https://doi.org/10.1101/2024.08.23.609373

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