May mineral composition trigger or limit the protein content in soybean (Glycine max (L.) Merrill) seeds? Insights from a survey on 95 varieties cultivated in Brazil

Montanha, G. S.; Perez, L. C.; Brandao, J. R.; de Camargo, R. F.; Tavares, T. R.; de Almeida, E.; Pereira de Carvalho, H. W. W. P.

doi:10.1101/2022.09.29.510200

bioRxiv · 10.1101/2022.09.29.510200

May mineral composition trigger or limit the protein content in soybean (Glycine max (L.) Merrill) seeds? Insights from a survey on 95 varieties cultivated in Brazil

Montanha, G. S.·Perez, L. C.·Brandao, J. R.·de Camargo, R. F.·Tavares, T. R.·de Almeida, E.·Pereira de Carvalho, H. W. W. P.

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Abstract

Background and AimsSoybean (Glycine max (L.) Merrill) stands out as the major source of protein and oil for human and animal nutrition. Nevertheless, the increase in soybean yield has been accompanied by a reduction in its protein content in the last few decades. Since this might be influenced by the elemental composition of the seeds, we herein aimed at determining the profile of mineral nutrients and protein of 95 soybean varieties broadly cultivated in Brazil, the worlds biggest soybean producer and exporter, to identify possible nutritional triggers for the protein content. MethodsEnergy dispersive fluorescence spectroscopy (EDXRF) was employed to determine the concentration of macro, i.e., (K), phosphorus (P), sulphur (S), calcium (Ca), and micronutrients, i.e., iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu). The protein content was evaluated in soybean seeds by the Dumas method. The correlational and clustering assessment between nutrients and protein were determined through both univariate and multivariate non-parametric tests. ResultsBoth protein and nutrient concentrations are not homogeneous across soybean seed varieties, and a clear positive association between protein and sulphur (S), zinc (Zn), and manganese (Mn) concentrations were observed. ConclusionThe recorded results suggest that sulphur (S), zinc (Zn), and manganese (Mn) are the limiting nutrients for higher protein content in soybean seeds.

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Montanha, G. S., Perez, L. C., Brandao, J. R., de Camargo, R. F., Tavares, T. R., de Almeida, E., Pereira de Carvalho, H. W. W. P.. 2022-10-01. May mineral composition trigger or limit the protein content in soybean (Glycine max (L.) Merrill) seeds? Insights from a survey on 95 varieties cultivated in Brazil. https://doi.org/10.1101/2022.09.29.510200

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