A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model

Haskey, N.; Ye, J.; Estaki, M.; Verdugo-Meza, A.; Barnett, J. A.; Yousefi, M.; Birnie, B.; Gruenheid, S.; Ghosh, S.; Gibson, D. L.

doi:10.1101/2021.09.27.462042

bioRxiv · 10.1101/2021.09.27.462042

A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model

Haskey, N.·Ye, J.·Estaki, M.·Verdugo-Meza, A.·Barnett, J. A.·Yousefi, M.·Birnie, B.·Gruenheid, S.·Ghosh, S.·Gibson, D. L.

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Abstract

The Mediterranean diet (MD) is a health-promoting, moderate fat diet (~ 40% energy). It is not known if the blend of fats found in the MD contribute to the beneficial protective effects. We compared the MD fat blend (high monounsaturated, 2:1 n-6:n-3 polyunsaturated and moderate saturated fat) to isocaloric diets composed with corn oil (CO, n-6 polyunsaturated-rich), olive oil (high monounsaturated-rich) or milk fat (MF, saturated-rich) on spontaneous colitis development in Muc2-/- mice. The MD resulted in lower clinical and histopathological scores, and induced tolerogenic CD103+CD11b+ dendritic, Th22 and IL-17+IL-22+ cells important for intestinal barrier repair. MD also reduced attendant insulin resistance and a shift to a higher health-promoting gut microbes including Lactobacillus B. animalis and Muribaculaceae, whereas CO showed higher prevalence of mucin-degraders (Akkermansia muciniphila) and colitis promoters (Enterobacteriaceae). Our findings suggest that the MD fat blend could be recommended as a maintenance diet for colitis.

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Haskey, N., Ye, J., Estaki, M., Verdugo-Meza, A., Barnett, J. A., Yousefi, M., Birnie, B., Gruenheid, S., Ghosh, S., Gibson, D. L.. 2021-09-29. A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model. https://doi.org/10.1101/2021.09.27.462042

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