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bioRxiv · 10.64898/2026.11.05.764477

Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam

Nguyen, T.-L.·Tran, H.-D.
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Abstract

A microalgal strain from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam, was examined by light microscopy and three molecular markers. Thirty-six cells from 22 micrographs measured 6.68-12.74 by 3.49-4.79 micrometres. Homologous phylogenetic windows comprised 550 nucleotides for SSU rDNA, 711 for ITS and 1,170 for rbcL. CCAH0201, P1 and Tetratostichococcus jenerensis SAG 2138 shared the SSU 3-prime sequence, whereas ITS and rbcL placed CCAH0201 with P1 (SH-aLRT/UFBoot 87/94 and 96.9/99). CCAH0201 differed from P1 at 22/711 ITS and 4/1,170 rbcL sites. Structure-guided ITS2 comparison identified one strict CBC, one two-sided change from a wobble pair and three hemi-CBCs relative to P1. CCAH0201 is thus identified as Tetratostichococcus sp. in a lineage close to P1.

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Nguyen, T.-L., Tran, H.-D.. 2026-11-06. Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam. https://doi.org/10.64898/2026.11.05.764477

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