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bioRxiv · 10.64898/2026.11.06.764861

Identification and characterization of new P. aeruginosa phages using a surface receptor platform

Tran, V. N.·Lahane, T. S.·Qaderi, I.·Harvey, H.·Burrows, L. L.
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Abstract

Bacteriophages are of increasing interest for their potential use as alternatives to antibiotics for treating drug-resistant infections. Phage steering, specifically receptor-based steering, involves use of phages that bias the development of resistance in bacteria towards phenotypes such as reduced fitness. Phage therapy typically involves administering cocktails of phages with different targets to reduce the likelihood of resistance emerging. To ensure effective steering and cocktail design, there is a need to identify the receptors used by phages of interest, and to isolate phages that use diverse receptors. Here we describe a SURFAce Receptor (SURFAR) platform consisting of a set of defined Pseudomonas aeruginosa strains and mutants for rapid characterization of phage receptors. This platform can also be used to enrich for and isolate diverse phages from environmental samples, yielding novel phage genomes from wastewater. Using the SURFAR platform, two new phages were isolated and characterized: Dalewood, which targets both flagella and the conserved LPS outer core, and Bowman, a biofilm targeting phage capable of supressing growth of a subset of carbapenem-resistant clinical isolates. This tool helps to standardize the laborious but important task of phage receptor characterization, with the goal of expanding the known repertoire of P. aeruginosa phage receptors and phages.

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Tran, V. N., Lahane, T. S., Qaderi, I., Harvey, H., Burrows, L. L.. 2026-11-07. Identification and characterization of new P. aeruginosa phages using a surface receptor platform. https://doi.org/10.64898/2026.11.06.764861

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