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bioRxiv · 10.64898/2026.05.20.726740

Molecular and spatial characterization of baicalin from Scutellaria baicalensis hairy root culture

Fedorova, A. M.·Milentyeva, I. S.·Asyakina, L. K.·Prosekov, A. Y.
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Abstract

This study presents the structural verification of baicalin isolated from a hydroethanolic extract of an in vitro Scutellaria baicalensis root culture using X-ray diffraction analysis and a set of NMR spectroscopy techniques. The crystalline molecular structure of the sample was found to correspond to baicalin. The 1H, 13C{1H}, 2D 1H1H-COSY, 1H13C-HSQC, 1H13C-HMBC spectra confirmed that the chemical shifts, signal multiplicities, integral intensities, and spin-spin coupling constants were fully consistent with the structure of the target compound. Minor impurity signals were detected in the aliphatic region of the spectra, with a total content not exceeding 5 mol%. These results confirm the high purity and structural individuality of baicalin, a biologically active flavonoid glycoside of considerable interest.

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Fedorova, A. M., Milentyeva, I. S., Asyakina, L. K., Prosekov, A. Y.. 2026-05-22. Molecular and spatial characterization of baicalin from Scutellaria baicalensis hairy root culture. https://doi.org/10.64898/2026.05.20.726740

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