Biology subjects

Milentyeva, I. S.

Publications and source records attributed to Milentyeva, I. S..

3 recordsLinked to original sources

bioRxiv2023

Effect of Mangiferin Isolated from Hedysarum neglectum L. Root Culture on Fat Accumulation in Experiments In Vivo

Plant polyphenols possess diverse medicinal and therapeutic properties. For instance, they can prevent such age-related diseases as stroke, heart attack, type II diabetes, and their various pathological complications. Mangiferin is a polyphenolic compound with a C-glycosylxanthone structure. This biologically active substance is known for its antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and immunomodulatory properties. This research features mangiferin isolated from Hedysarum neglectum L. root culture and its effect on fat accumulation in Caenorhabditis elegans N2 Bristol. A set of experiments in vivo proved that mangiferin was able to inhibit fat accumulation in the model organism. The optimal concentration was determined as 50 m: it provided 44.5% fluorescence.

plant biology↗

bioRxiv2023

Effect of quercetin and kaempferol isolated from the Ginkgo biloba L. suspension culture extract on lipofuscin accumulation

Lipofuscin, known as an age pigment, is an autofluorescent lipopigment formed by lipids, metals, and misfolded proteins that accumulates in nerve cells, heart muscle cells, and skin. Individual bioactive compounds obtained from medicinal plant extracts reduce the level of lipofuscin in C. elegans nematodes in in vivo experiments. We aimed to study the effect of quercetin and kaempferol isolated from the in vitro suspension culture extracts of Ginkgo biloba L. on the accumulation of lipofuscin. According to the results, 200 M concentrations of these bioactive compounds showed the maximum decrease in lipofuscin levels in C. elegans N2 Bristol during their 15-day cultivation, compared to the control. Thus, our study showed that quercetin and kaempferol are able to reduce this age pigment in in vivo experiments.

developmental biology↗

bioRxiv2022

The effect of biohanin A isolated from the callus culture of meadow clover (Trifolium pratense L.) on the life expectancy and survival of Caenorhabditis elegans

Oxidative stress, in which healthy aging is limited, is a problem, since in the process there is an uncontrolled production of reactive radicals that negatively affect cells. Heat stress is closely related to oxidative stress, as it stimulates the production of radicals. Meadow clover (Trifolium pratense L.) is a promising source of isoflavonoids that have various positive effects on the body. The aim of the work is to study the effect of biohanin A, isolated from Trifolium pratense L. callus cultures, on the life expectancy of the C. elegans model organism, as well as its survival under oxidative and thermal stress. It was found that biohanin A increased the lifespan of C. elegans worms. The best concentration was determined - 100 microns, at which the survival rate of nematodes after 61 days of incubation was 16.0%. After 48 hours, the survival rate of worms was the highest (87.1%) at a concentration of biohanin A of 200 microns. Other concentrations 10, 50, 100 (81,2, 83,0, 82,4 %) they also showed a higher survival rate compared to the control (74.0%). In addition, the survival rate under heat stress of C. elegans was higher by 12.0; 8.4; 4.0% compared with the control. Thus, the results show the antioxidant potential of biohanin A from plant material under oxidative and thermal stress. The substance also had a positive effect on the lifespan of C. elegans.

plant biology↗