Shared book reading promotes experience-dependent autonomic synchrony in parent-preterm infant dyads

Lavezzo, L.; Meuleman, B.; Grandjean, D.; Gentaz, E.; Delplanque, S.; Ceravolo, L.; Scilingo, E. P.; Hüppi, P.; Barcos-Munoz, F.; Borradori-Tolsa, C.; Nardelli, M.; Filippa, M.

doi:10.64898/2026.05.19.726001

bioRxiv · 10.64898/2026.05.19.726001

Shared book reading promotes experience-dependent autonomic synchrony in parent-preterm infant dyads

Lavezzo, L.·Meuleman, B.·Grandjean, D.·Gentaz, E.·Delplanque, S.·Ceravolo, L.·Scilingo, E. P.·Hüppi, P.·Barcos-Munoz, F.·Borradori-Tolsa, C.·Nardelli, M.·Filippa, M.

View original source ↗Export metadata ↓

Abstract

Preterm birth is associated with alterations in early caregiver-infant regulation, with potential consequences for socio-emotional and physiological development. However, the mechanisms through which early interactional experience shapes these processes remain unclear. Here, we tested whether a structured dyadic intervention could modify co-regulatory dynamics across physiological, behavioral, and relational levels. Fifty-four 7-month-old preterm infants and their parents were assigned to either a shared book reading intervention (n = 22) or an active control condition based on a shared building activity (n = 32) and compared with 39 full-term infants. The intervention consisted of an 8-week program of shared book reading, designed to structure parent-infant interaction. Physiological synchrony was assessed at the dyadic level, alongside infants autonomic regulation and cardiovascular signal complexity. Behavioral engagement and parental attachment representations were also evaluated. Results showed that mother-infant physiological synchrony emerged selectively within the interactional context trained by the intervention and only in the intervention group. This context-specific synchrony was accompanied by modulation of vagal activity and increased cardiovascular complexity in preterm infants, consistent with enhanced flexibility of autonomic control. At the behavioral and relational levels, intervention infants showed increased initiating joint attention, while parents reported higher secure attachment. These findings support a model of experience-dependent early synchrony, in which repeated dyadic interaction through shared book reading shapes the coupling between interpersonal coordination and individual physiological regulation. By linking synchrony, autonomic flexibility, and social engagement, this study identifies a mechanism through which early caregiving experience can organize developmental trajectories following prematurity.

Explore related subjects

developmental biology Preprint

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Lavezzo, L., Meuleman, B., Grandjean, D., Gentaz, E., Delplanque, S., Ceravolo, L., Scilingo, E. P., Hüppi, P., Barcos-Munoz, F., Borradori-Tolsa, C., Nardelli, M., Filippa, M.. 2026-05-20. Shared book reading promotes experience-dependent autonomic synchrony in parent-preterm infant dyads. https://doi.org/10.64898/2026.05.19.726001

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Hyaluronic acid dynamics are regulated by neural crest cells and NEDD4 in the developing cardiac outflow tract valves

The extracellular matrix (ECM) is essential for cardiac valve development, with hyaluronic acid (HA) playing a critical role in cardiac cushion formation. While HA is required for atrioventricular valve development, its role in semilunar valve formation remains poorly understood. The ubiquitin ligase NEDD4 is essential for cardiac outflow tract development, particularly within cardiac neural crest cells, however its role in semilunar valve development has been unexplored. Here, we demonstrate that neural crest-specific deletion of NEDD4 in mice disrupts outflow tract valve development, resulting in abnormal leaflet number and morphology. Analysis of valve ECM revealed a specific reduction in HA within the neural crest-derived valve mesenchyme, while other ECM components such as fibronectin and versican remained unaffected. In contrast, deletion of NEDD4 in endocardium-derived valve mesenchyme did not alter HA abundance. Using NEDD4-deficient neural crest cells, we further demonstrate a cell-autonomous requirement for NEDD4 in regulating HA abundance, with in vitro evidence supporting impaired HA synthesis rather than increased turnover of HA in the ECM. Our findings identify a previously unrecognised role for NEDD4 in regulating HA synthesis in cardiac neural crest cells, revealing a cell-type-specific mechanism governing semilunar valve development and highlighting distinct cellular requirements for ECM regulation across cardiac valve populations.

developmental biology↗

bioRxiv2026

Cxcr2 is required for ductal outgrowth and shapes the stromal microenvironment in postnatal mammary gland development

Mammary gland development requires coordinated signaling between epithelial and stromal compartments. While CXC chemokine receptor 2 (CXCR2) is known to regulate inflammatory and tissue remodeling processes in disease, including breast cancer, its role in mammary gland morphogenesis is not well understood. Here, we examined postnatal mammary gland development in germline Cxcr2 knockout mice. Loss of Cxcr2 resulted in delayed ductal outgrowth, reduced epithelial area, and altered stromal architecture, including decreased adipocyte size and increased collagen deposition, despite preserved branching organization. These defects occurred in the context of severe systemic immune dysregulation, marked by expansion of granulocytic populations and loss of adaptive immune cells. Together, our findings indicate that CXCR2 indirectly regulates mammary gland development through coordination between the epithelium and stroma, rather than acting through epithelial cell-intrinsic mechanisms. Because the same myeloid expansion, immune suppression, and collagen-rich stromal remodeling that CXCR2 controls during normal development are hallmarks of the pro-tumorigenic breast microenvironment, these findings establish CXCR2 as a regulator of the immune and stromal landscape of the mammary gland and provide a developmental framework for understanding its contributions to the breast tumor microenvironment.

developmental biology↗

bioRxiv2026

Endogenous Mechanical Reporters Encode the Global Mechanical Landscape of Morphogenesis

Developmental systems usually comprise multiple genetically programmed morphogenetic events within a mechanically continuous environment, yet how individual processes interact and contribute to the overall mechanical landscape remains unknown. Here, we introduce the concept of mechanical reporter: endogenous tissues function as in situ probes of the embedded mechanical landscape. We exemplified cephalic furrow (CF) migration as a mechanical reporter in gastrulating Drosophila. Using optogenetics and genetics, we revealed that CF displacement reflects the combined effect of both force and tissue mechanical properties. By incorporating condition-specific mechanical landscape into a continuum minimal-response model, we developed a framework to quantitatively predict the reporter dynamics. Using different genetic mutants, we successfully decoded changes in the mechanical landscape from CF dynamics in gastrulating Drosophila. Overall, we established a predictive framework that uses CF dynamics to infer changes in the global mechanical landscape. Beyond this, the concept of mechanical reporter provides a general strategy for systematically understanding and decoding complex mechanical environments in living systems.

developmental biology↗