LAT condensation gates PLCγ1 activation via bimodal LAT phosphorylation

Ren, H.; Lee, H.-R.; Omar, Y. A. D.; DeGrandchamp, J. B.; Hung, C.-L.; Eisen, T. J.; Stamou, D.; Kuriyan, J.; Chakraborty, A. K.; Groves, J. T.

doi:10.64898/2026.04.29.721478

bioRxiv · 10.64898/2026.04.29.721478

LAT condensation gates PLCγ1 activation via bimodal LAT phosphorylation

Ren, H.·Lee, H.-R.·Omar, Y. A. D.·DeGrandchamp, J. B.·Hung, C.-L.·Eisen, T. J.·Stamou, D.·Kuriyan, J.·Chakraborty, A. K.·Groves, J. T.

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Abstract

T cells can respond to even a single molecular binding event of antigen to a TCR. A key step in the TCR signaling pathway that definitively exhibits this single molecule response is the initiation of calcium influx by activation of PLC{gamma}1 in the LAT protein condensate. Here, we describe detailed kinetic measurements examining how protein condensation of LAT regulates activation of PLC{gamma}1 using a reconstituted membrane system. The results reveal that membrane recruitment of PLC{gamma}1 is tightly controlled by the LAT phosphorylation state, with no measurable independent recruitment to PIP2 or PIP3 lipids via the PLC{gamma}1 PH domains. We further observe PLC{gamma}1 is rapidly activated by membrane-associated kinase upon recruitment, irrespective of the LAT condensation state. These studies also revealed a crosstalk mechanism in which the TEC family kinases responsible for PLC{gamma}1 activation also phosphorylate LAT. This interaction establishes a positive feedback loop in LAT phosphorylation, mediated through LAT condensation, which drives a bimodal LAT phosphorylation response to TCR activation. Kinetic modeling reveals how this LAT phosphorylation response can cooperatively gate PLC{gamma}1 activation from a single TCR. These results suggest the LAT condensate facilitates both signal amplification and noise suppression in PLC{gamma}1 activation through a bimodal switch affecting LAT phosphorylation. Significance StatementT cells are sensitive sensors capable of detecting and responding to trace amounts of foreign antigen. Understanding how they achieve such sensitivity while maintaining accurate antigen discrimination remains a key challenge. Here, through detailed kinetic measurements of PLC{gamma}1 activation, we identify a cross reactivity in which kinases responsible for PLC{gamma}1 phosphorylation also phosphorylate LAT. This creates a bimodal switch controlling LAT phosphorylation levels, which gates PLC{gamma}1 activation from single TCR signals. We suggest this mechanism plays a key role in the signal amplification and noise suppression required for T cells to detect single antigen molecules.

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Ren, H., Lee, H.-R., Omar, Y. A. D., DeGrandchamp, J. B., Hung, C.-L., Eisen, T. J., Stamou, D., Kuriyan, J., Chakraborty, A. K., Groves, J. T.. 2026-05-01. LAT condensation gates PLCγ1 activation via bimodal LAT phosphorylation. https://doi.org/10.64898/2026.04.29.721478

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