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bioRxiv · 10.64898/2026.04.17.719198

Metabolic fingerprinting of 17 Brassicaceae species across three tissues

Wolters, F. C.·Woldu Semere, T.·Schranz, M. E.·Medema, M. H.·Bouwmeester, K.·van der Hooft, J. J. J.
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Abstract

Plants produce the most diverse blends of specialized metabolites on earth. Natural products derived from plants are valuable resources for drug development, food chemistry, and crop resistance breeding. Phenotypes of specialized metabolite profiles can be captured by untargeted mass-spectrometry across species phylogeny, tissues, and genotypes. Here, we collected metabolic fingerprints of 17 Brassicaceae species across three tissues (paired leaf and root; flower) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive and negative ionization mode. Corresponding metadata has been refined for reuse according to ReDU guidelines, and for integration with public genomic and transcriptomic data. Standardization of in vitro growth conditions, and data processing workflows enables integration of acquired raw and processed data across platforms for single- and multi-omics analysis. Further, the inclusion of tissue-specific metabolic profiles across ploidy levels, as well as across crop species and wild relatives, makes this dataset a valuable resource for natural product discovery.

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Wolters, F. C., Woldu Semere, T., Schranz, M. E., Medema, M. H., Bouwmeester, K., van der Hooft, J. J. J.. 2026-04-21. Metabolic fingerprinting of 17 Brassicaceae species across three tissues. https://doi.org/10.64898/2026.04.17.719198

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