Gliomas preferentially develop within the action-mode network

Cui, W.; Zhu, J.; Yan, Z.; Ren, J.; Marek, S.; Stoecklein, V. M.; Jiang, T.; Bao, H.; Fang, S.; Stoecklein, S.; Cai, Z.; Fu, X.; Gordon, E. M.; Wang, D.; Wang, Y.; Dosenbach, N. U. F.; Liu, H.

doi:10.64898/2026.01.05.697608

bioRxiv · 10.64898/2026.01.05.697608

Gliomas preferentially develop within the action-mode network

Cui, W.·Zhu, J.·Yan, Z.·Ren, J.·Marek, S.·Stoecklein, V. M.·Jiang, T.·Bao, H.·Fang, S.·Stoecklein, S.·Cai, Z.·Fu, X.·Gordon, E. M.·Wang, D.·Wang, Y.·Dosenbach, N. U. F.·Liu, H.

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Abstract

Gliomas tend to arise in specific brain regions and may integrate into functional circuits, suggesting they could be regulated by brain activity. However, it remains unclear whether glioma growth is related to system-level brain networks. Analyzing neuroimaging data from three datasets including 1,310 patients with cerebral gliomas, we identified and replicated a functionally connected glioma network, which overlaps with the action-mode network (AMN), somatomotor network (SMN), and action-related subcortical regions. Resting-state functional connectivity (RSFC) of the AMN successfully predicted the location of glioma occurrence in two independent datasets with complex tumor distributions. Remarkably, no patient had a glioma entirely outside the AMN, and over 89% of patients exhibited gliomas with at least 50% overlap with the network. Moreover, the spatial overlap between glioma location and the AMN demonstrated significant prognostic value in survival analyses, with higher AMN-tumor overlap associated with poorer overall survival. Notably, the acetylcholine transporter, a key player in glioma pathogenesis that drives transcriptional reprogramming, showed an expression pattern overlapping with the AMN. Meta-analytic annotations further linked the glioma network to processes of action initiation, execution, and feedback. These findings indicate that gliomas preferentially arise in circuits involved in action and highlight the central role of the AMN in glioma pathophysiology and growth.

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Cui, W., Zhu, J., Yan, Z., Ren, J., Marek, S., Stoecklein, V. M., Jiang, T., Bao, H., Fang, S., Stoecklein, S., Cai, Z., Fu, X., Gordon, E. M., Wang, D., Wang, Y., Dosenbach, N. U. F., Liu, H.. 2026-01-05. Gliomas preferentially develop within the action-mode network. https://doi.org/10.64898/2026.01.05.697608

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