Evolution of linkage and genome expansion in protocells

Abstract

Chromosomes are likely to have followed unlinked genes in early evolution. Genetic linkage reduces the assortment load and intragenomic conflict in reproducing protocell models to the extent that chromosomes can go to fixation even if chromosomes suffer from a replicative disadvantage, relative to unlinked genes, proportional to their length. Here we show that chromosomes spread within protocells even if recurrent deleterious mutations affecting replicating genes (as ribozymes) are taken into account. Dosage effect selects for optimal genomic composition within protocells that carries over to the genic composition of emerging chromosomes. Lacking an accurate segregation mechanism protocells continue to benefit from the stochastic corrector principle (group selection of early replicators), but now at the chromosome level. A remarkable feature of this process is the appearance of multigene families (in optimal genic proportions) on chromosomes. An added benefit of chromosome formation is an increase in the selectively maintainable genome size (number of different genes), primarily due to the marked reduction of the assortment load. This result complements the established benefit conferred by chromosomes on protocells allowing for the fixation of highly specific and efficient enzymes.