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bioRxiv · 10.1101/573618

Toxoplasma controls host cyclin E expression through the use of a novel MYR1-dependent effector protein, HCE1

Panas, M. W.·Naor, A.·Cygan, A.·Boothroyd, J.
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Abstract

Toxoplasma gondii is an obligate intracellular parasite that establishes a favorable environment in the host cells in which it replicates. We have previously reported that it uses MYR-dependent translocation of dense granule proteins to elicit a key set of host responses related to the cell cycle, specifically E2F transcription factor targets including cyclin E. We report here the identification of a novel Toxoplasma effector protein that is exported from the parasitophorous vacuole in a MYR1-dependent manner and localizes to the hosts nucleus. Parasites lacking this inducer of Host Cyclin E (HCE1) are unable to modulate E2F transcription factor target genes and exhibit a substantial growth defect. Immunoprecipitation of HCE1 from infected host cells shows that HCE1 efficiently binds elements of the cyclin E regulatory complex, DP1 and its partners E2F3 and E2F4. Expression of HCE1 in Neospora caninum, or in uninfected HFFs, shows localization of the expressed protein to the host nuclei and strong cyclin E up-regulation. Thus, HCE1 is a novel effector protein that is necessary and sufficient to impact the E2F-axis of transcription resulting in co-opting of host functions to Toxoplasmas advantage.

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Panas, M. W., Naor, A., Cygan, A., Boothroyd, J.. 2019-03-11. Toxoplasma controls host cyclin E expression through the use of a novel MYR1-dependent effector protein, HCE1. https://doi.org/10.1101/573618

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