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bioRxiv · 10.1101/505297

Introducing RpsA Point Mutation Δ438A or D123A into the Chromosome of M. tuberculosis Confirms its Role in Causing Resistance to Pyrazinamide

Shi, W.·Cui, P.·Niu, H.·Zhang, S.·Tonjum, T.·Zhu, B.·Zhang, Y.
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Abstract

Pyrazinamide (PZA) is a unique frontiline drug for shortening tuberculosis treatment, but its mechanisms of action are elusive. We previously identified RpsA as a target of PZA and found an alanine deletion at position 438 ({Delta}438A) in RpsA associated with PZA resistance, but its role in PZA resistance is controversial. Here, we introduced RpsA mutation {Delta}438A or D123A into M. tuberculosis chromosome and demonstrated that the introduced RspA mutations are indeed responsible for PZA resistance.

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Shi, W., Cui, P., Niu, H., Zhang, S., Tonjum, T., Zhu, B., Zhang, Y.. 2019-01-10. Introducing RpsA Point Mutation Δ438A or D123A into the Chromosome of M. tuberculosis Confirms its Role in Causing Resistance to Pyrazinamide. https://doi.org/10.1101/505297

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