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bioRxiv · 10.1101/386110

Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold

Steinegger, M.·Mirdita, M.·Soding, J.
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Abstract

The open-source de-novo Protein-level assembler Plass (https://plass.mmseqs.org) assembles six-frame-translated sequencing reads into protein sequences. It recovers 2 to 10 times more protein sequences from complex metagenomes and can assemble huge datasets. We assembled two redundancy-filtered reference protein catalogs, 2 billion sequences from 640 soil samples (SRC) and 292 million sequences from 775 marine eukaryotic metatranscriptomes (MERC), the largest free collections of protein sequences.

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Steinegger, M., Mirdita, M., Soding, J.. 2018-08-07. Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold. https://doi.org/10.1101/386110

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