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bioRxiv · 10.1101/327999

TLR3-TRIF pathway activation by Neospora caninum RNA enhances infection control

Miranda, V. d. S.·Franca, F. B. F.·da Costa, M. S.·Silva, V. R. S.·Mota, C. M.·Barros, P. d. S. C.·Parreira, K. S.·Santiago, F. M.·Mineo, J. R.·Mineo, T. W.
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Abstract

Neospora caninum is a protozoan parasite closely related to Toxoplasma gondii and has been studied for causing neuromuscular disease in dogs and abortions in cattle. It is recognized as the major cause of economic losses in bovine products. In that sense, this study aimed to evaluate the role of TLR3-TRIF dependent resistance against N. caninum infection. We observed that TLR3-/- and TRIF-/- mice presented higher parasite burden, increased inflammatory lesions and reduced production of IL-12p40, TNF, IFN-{gamma}, and NO. Differently from T. gondii, N. caninum tachyzoites and its RNA recruited TLR3 and IRF3 to the parasitophorous vacuole (PV). We observed that N. caninum upregulated the expression of TRIF in macrophages, which by its turn upregulated IFN- and IFN-{beta} in the presence of the parasite. Furthermore, TRIF-/- infected macrophages produced lower levels of IL-12p40 and IFN- replacement was able to completely restore the production of this key cytokine. Our results have shown that TLR3-TRIF signaling pathway enhances resistance against N. caninum infection, since it improves Th1 immune responses that control parasitism and tissue inflammation, which are hallmarks of the disease.

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Miranda, V. d. S., Franca, F. B. F., da Costa, M. S., Silva, V. R. S., Mota, C. M., Barros, P. d. S. C., Parreira, K. S., Santiago, F. M., Mineo, J. R., Mineo, T. W.. 2018-05-22. TLR3-TRIF pathway activation by Neospora caninum RNA enhances infection control. https://doi.org/10.1101/327999

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