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bioRxiv · 10.1101/2025.08.23.671908

GCAD: a Computational Framework for Mammalian Genetic Program Computer-Aided Design

Abstract

Genetic programs can direct living systems to perform diverse, pre-specified functions. As the library of parts available for building such programs continues to expand, computation-guided design is increasingly helpful and necessary. Predictive models aid the challenging design process, but iterative simulation and experimentation are intractable for complex functions. Computer-aided design accelerates this process, but existing tools do not yet capture the behavior of mammalian-specific parts and population-level effects needed for mammalian synthetic biologists. To address these needs, we developed a framework for mammalian genetic program computer-aided design. Starting with a user-defined design specification to quantify circuit performance, the framework uses a genetic algorithm to search through possible designs. Circuit space is defined by a library of experimentally characterized parts and dynamical systems models for gene expression in a heterogeneous cell population. We developed this genetic algorithm using a directed graph-based formulation with biologically constrained rules to explore regulatory connections and parts. We evaluated the framework for design problems of varying complexity, including programs we describe as an amplifier, signal conditioner, and pulse generator, demonstrating that the algorithm can successfully find optimal circuit designs. Finally, we experimentally evaluated selected circuits, demonstrating the path from a predicted circuit design to experimental testing and highlighting the importance of characterization for enabling predictive design. Overall, this framework establishes general approaches that can be refined and expanded, accelerating the design and implementation of mammalian genetic programs.

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BibTeXRIS

Dreyer, K. S., Nguyen, A. V., Bora, G. G., Redus, L. E., Edelstein, H. I., Garcia, J., Anastasia, E., Dray, K. E., Leonard, J. N., Mangan, N. M.. 2025-08-23. GCAD: a Computational Framework for Mammalian Genetic Program Computer-Aided Design. https://doi.org/10.1101/2025.08.23.671908

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