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bioRxiv · 10.1101/2025.04.30.651002

Bacteriophage-Loaded Microneedle Patches for Targeted andMinimally Disruptive Foodborne Pathogen Decontamination

Prasad, A.·Khan, S.·Arshad, F.·Sidhu, H.·Jackson, K.·MacLachlan, R.·Kvitka, E.·Grignano, V.·Mann, H.·Filipe, C.·Hosseinidoust, Z.·Didar, T.
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Abstract

Antibacterial additive use has surged due to rising incidences of food contamination, despite concerns over antibiotic resistance. Bacteriophage (bacterial viruses) represent a unique and promising opportunity as antibacterial agents, offering targeted bacterial lysis while being food safe. However, their commercial success has been limited by the significant diffusion barriers they face within food, preventing effective delivery at contamination sites. Here, we introduce bacteriophage-loaded microneedle patches that enable targeted phage delivery directly within food, eliminating internal pathogens in a minimally disruptive manner. The application of microneedles within food is first explored. The platform is then substantiated by comparing performance in raw beef and cooked chicken, where we achieved up to 3-logs reduction in Escherichia coli, thus providing complete decontamination according to regulatory limits. In contrast, conventional surface application of the same phage failed to provide significant decontamination. To ensure broad applicability, phage cocktails were also loaded into microneedles to demonstrate polymicrobial decontamination against other common food contaminants including Salmonella. This platform can also be adapted to extend food shelf-life by targeting spoilage-inducing bacteria.

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Prasad, A., Khan, S., Arshad, F., Sidhu, H., Jackson, K., MacLachlan, R., Kvitka, E., Grignano, V., Mann, H., Filipe, C., Hosseinidoust, Z., Didar, T.. 2025-05-04. Bacteriophage-Loaded Microneedle Patches for Targeted andMinimally Disruptive Foodborne Pathogen Decontamination. https://doi.org/10.1101/2025.04.30.651002

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