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Biology subjects

Didar, T.

Publications and source records attributed to Didar, T..

2 recordsLinked to original sources

Food-activated Microneedle Sensor for Real-time, Colorimetric Spoilage Monitoring of Pre-packaged Food

At a time of growing food insecurity, developing technologies to reduce food waste is critical. We report an inexpensive, colorimetric spoilage sensor for real-time food product assessment. The sensor is composed of dehydrated gelatin microneedles that exhibit high mechanical integrity in their base state. However, once exposed to fluid-rich food environments, they rapidly transition to a hydrogel sensing state. Food-derived anthocyanins embedded within these microneedles enable pH-based spoilage monitoring. When applied to sealed fish products, these microneedles non-destructively penetrate through packaging and are rehydrated by the underlying fish matrix. As the product ages, a defined colour shift occurs, demonstrating strong correlation with quantitative spoilage markers. When applied to unsealed fish products for rapid testing, the large microneedle sensing interface enables accelerated colorimetric sensing. Finally, successful fresh versus spoiled categorization of smartphone-acquired images of the sensor using machine learning removes readout ambiguity, empowering consumers with independent real-time product monitoring.

molecular biology↗

Bacteriophage-Loaded Microneedle Patches for Targeted andMinimally Disruptive Foodborne Pathogen Decontamination

Antibacterial additive use has surged due to rising incidences of food contamination, despite concerns over antibiotic resistance. Bacteriophage (bacterial viruses) represent a unique and promising opportunity as antibacterial agents, offering targeted bacterial lysis while being food safe. However, their commercial success has been limited by the significant diffusion barriers they face within food, preventing effective delivery at contamination sites. Here, we introduce bacteriophage-loaded microneedle patches that enable targeted phage delivery directly within food, eliminating internal pathogens in a minimally disruptive manner. The application of microneedles within food is first explored. The platform is then substantiated by comparing performance in raw beef and cooked chicken, where we achieved up to 3-logs reduction in Escherichia coli, thus providing complete decontamination according to regulatory limits. In contrast, conventional surface application of the same phage failed to provide significant decontamination. To ensure broad applicability, phage cocktails were also loaded into microneedles to demonstrate polymicrobial decontamination against other common food contaminants including Salmonella. This platform can also be adapted to extend food shelf-life by targeting spoilage-inducing bacteria.

bioengineering↗