Search bioRxiv⌕ Search

bioRxiv · 10.1101/2025.04.15.648814

OPTIMIZING AN EXOTENDON AT AN INCREASED RUNNING SPEED

Abstract

The exotendon is a passive device that reduces the energetic cost of running at 2.7 m/s, but its potential benefits at higher speeds remain unknown. Experimental testing is challenging because of the wide range of conditions that must be tested. Here, we use muscle-driven simulations to overcome this challenge and inform exotendon design. We validated a simulation framework that estimates changes in energy expenditure, body kinematics, and muscle activations when simulated subjects run with and without an exotendon. Simulations of people running at 4 m/s with the exotendon that saved energy at 2.7 m/s predicted a 10% reduction in energy cost compared to natural running. We then performed simulations of 25 designs and found that many of the designs saved energy. A longer, stiffer exotendon yielded slightly greater energy savings (12%). Longer and more compliant exotendons offered little savings. We plan to test a limited set of our simulation predictions in an experiment to evaluate their accuracy and assess how an exotendon impacts running performance at 4 m/s. The purpose of this paper is to present the simulation results and to make predictions about the performance of the runner-exotendon system in experiments. This paper has been posted before the experiments have begun to avoid informing the predictions from the experimental results.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Stingel, J., Bianco, N. A., Ong, C., Hicks, J. L., Delp, S.. 2025-04-21. OPTIMIZING AN EXOTENDON AT AN INCREASED RUNNING SPEED. https://doi.org/10.1101/2025.04.15.648814

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

Single-Component Photo-Crosslinked Lipoic Acid Functionalized Mucin Hydrogels: Dynamic Mucus Mimetics for Antiviral Protection

Native mucus protects epithelial surfaces through viscoelastic networks of disulfide-linked mucins. However, commercially available mucins often fail to reconstitute comparable mechanical and barrier properties, while synthetic and hybrid mucus mimetics require polymeric crosslinkers that may alter mucus-like composition. Inspired by the native architecture, we directly functionalized porcine gastric and bovine submaxillary mucins with -lipoic acid to develop a single-component, photo-crosslinkable hydrogels. Upon UV irradiation (365 nm), the modified mucins rapidly form disulfide-crosslinked networks without added photo-initiator/ polymeric crosslinkers, two orders of magnitude faster as compared with PEG-SH approaches. The resulting mucin-lipoic acid (MUC-LA) hydrogels exhibit mucus-relevant properties, including tunable viscoelasticity, redox responsiveness, self-healing, and size-dependent restrictions on nanoparticle transport. In transwell-based infection models, MUC-LA hydrogels (2-3%) mimicking the healthy airway mucus (G' {approx} 10-20 Pa) suppress epithelial infection by SARS-CoV-2 and influenza A virus. Crosslinked MUC-LA provides greater protection against SARS-CoV-2 than the non-crosslinked, whereas both formulations suppress influenza A infection, suggesting distinct contributions from network formation and mucin-associated interactions. These findings establish a dynamic, mucus-mimetic platform integrating rapid photo-gelation, controllable mechanics, selective transport, and antiviral barrier functionality. MUC-LA hydrogels offer opportunities to investigate mucosal transport and host-pathogen interactions, with potential applications in drug delivery, and as biomaterials.

bioengineering↗

A Fibrous Optofluidic Probe for Endovascular Sensing of Blood Oxygenation and Flow Velocity

Local hemodynamic information including blood oxygenation and flow velocity could guide vascular interventions, yet obtaining these parameters in deep, narrow vessels remains challenging. Here, we report a flexible, submillimeter-scale fibrous optofluidic probe for endovascular sensing of blood oxygenation and flow velocity. The probe integrates a polymer optical fiber for light transmission with a microfluidic tube for dye injection, enabling spectroscopic oximetry and fluorescence dilution-based velocimetry through a single device. Via vascular intervention, we demonstrate local hemodynamic sensing in rabbit arteries and angiography-guided deployment in canine carotid arteries. The probe captures local changes in blood oxygenation and flow velocity during vascular occlusion and reperfusion, revealing hemodynamic disturbances that are not reflected in peripheral monitors. These results illustrate the complementary information provided by local oximetry and velocimetry and establish a compact optofluidic platform for quantitative hemodynamic monitoring during vascular interventions.

bioengineering↗

Broad-spectrum in situ genetic reprogramming of native microbiomes

Complex microbial communities are found throughout terrestrial, aquatic, and animal-associated ecosystems. Genetic modification of discrete microbial taxa within native communities represents a powerful tool to study and augment microbiome functions. Herein, we develop Synthetic Phage Assisted Reprogrammable and Targetable Addition (SPARTA) as a one-step strategy for packaging, delivering, and integrating large synthetic DNA into a broad range of native microbiomes. SPARTA leverages a broad-spectrum synthetic phage particle for in situ genome engineering within complex soil, freshwater, and mammalian fecal microbiomes, reprogramming 59 native species across 16 families. By modulating SPARTA components, we further demonstrated tunability of SPARTA towards targeted microbiome population. Additionally, we built a novel companion tool to quantitatively identify engineered taxa, named 16S crRNA-guided inference of bacterial engineering sequencing or ScRibe-seq. The SPARTA-ScRibe-seq pipeline delivered and validated functional synthetic gene clusters in diverse native microbiomes while preserving the original microbial community structure. Together, this novel genome engineering framework constitutes an end-to-end programmable platform for rational and customizable reprogramming of native microbiomes.

bioengineering↗