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bioRxiv · 10.1101/2024.12.19.628843

Spatial Perturb-Seq: Single-Cell Functional Genomics within Intact Tissue Architecture

Shen, K.·Seow, W. Y.·Keng, C. T.·Lim, D.·Guo, K.·Meliani, A.·Muhammad Bin Hajis, I.·Chen, K. H.·Chew, W. L.
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Abstract

We develop Spatial Perturb-Seq, an in vivo CRISPR technology that interrogates multiple genes within single cells of intact tissues. We apply Spatial Perturb-Seq to screen risk genes for neurodegenerative diseases in the mouse brain, uncovering cell autonomous and cell-cell microenvironmental effects within the spatially intact tissue. Spatial Perturb-Seq functionally screens multiple genes in situ and in vivo, identifying candidate genes underlying dysregulated neuronal intercellular communication pathways in Lrp1 signalling and ephrin-Eph receptor interactions.

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Shen, K., Seow, W. Y., Keng, C. T., Lim, D., Guo, K., Meliani, A., Muhammad Bin Hajis, I., Chen, K. H., Chew, W. L.. 2024-12-20. Spatial Perturb-Seq: Single-Cell Functional Genomics within Intact Tissue Architecture. https://doi.org/10.1101/2024.12.19.628843

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