Tropical Forest Soil Microbiome Modulates Leaf Heat Tolerance More Strongly Under Warming than Ambient Conditions

Hernandes Villani, G.; Grullon-Penkova, I. F.; Bartz, P.; Masanga, J.; Lasky, J. R.; Reed, S. C.; Cavaleri, M. A.; Wood, T. E.; Bachelot, B.

doi:10.1101/2024.12.09.626610

bioRxiv · 10.1101/2024.12.09.626610

Tropical Forest Soil Microbiome Modulates Leaf Heat Tolerance More Strongly Under Warming than Ambient Conditions

Hernandes Villani, G.·Grullon-Penkova, I. F.·Bartz, P.·Masanga, J.·Lasky, J. R.·Reed, S. C.·Cavaleri, M. A.·Wood, T. E.·Bachelot, B.

View original source ↗Export metadata ↓

Abstract

Tropical forests are increasingly threatened by climate change. Yet, it is still unclear how tropical plants respond to increasing temperatures. Leaf heat tolerance (LHT) in tropical plants is often at its upper limit, suggesting that climate change might negatively impact tropical forests. We hypothesized that intraspecific variation in this leaf trait might be associated with changes in the soil microbiome, which might also respond to climate. Specifically, we hypothesized that warming would increase LHT through changes in the soil microbiome: this study combined an in-situ tropical warming experiment with a shade house experiment in Puerto Rico. The shade house experiment consisted of growing seedlings of Guarea guidonia, a dominant forest species, under different soil microbiome treatments (reduced arbuscular mycorrhizal fungi, reduced plant pathogens, reduced microbes, and unaltered) and soil inoculum from the field experiment. Heat tolerance was determined using chlorophyll fluorescence (FV/Fm) on individual seedlings in the field and on pooled seedlings by pot in the shade house. We sequenced soil fungal DNA to analyze the impacts of the field and shade house treatments on the soil microbiome. In the field, seedlings from ambient temperature plots showed higher FV/Fm values under high temperatures (0.648 at 46 {degrees}C and 0.067 at 52 {degrees}C) than seedlings from the warming plots (0.535 at 46 {degrees}C and 0.031 at 52 {degrees}C). In the shade house, the soil microbiome treatments, which significantly influenced the fungal community composition, significantly influenced LHT. Reduction in fungal pathogen abundance and diversity altered FV/Fm before T50 for seedlings grown with soil inoculum from the warming plots but after T50 for seedlings grown with soil inoculum from the ambient plots. Our findings emphasize that the soil microbiome might play an important role in modulating the impacts of climate change on plants. Understanding and harnessing this relationship might be vital for mitigating the effects of warming on tropical forests, emphasizing the need for further research on microbial responses to climate change.

Source connections

Published article ↗

Explore related subjects

ecology Preprint

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Hernandes Villani, G., Grullon-Penkova, I. F., Bartz, P., Masanga, J., Lasky, J. R., Reed, S. C., Cavaleri, M. A., Wood, T. E., Bachelot, B.. 2024-12-10. Tropical Forest Soil Microbiome Modulates Leaf Heat Tolerance More Strongly Under Warming than Ambient Conditions. https://doi.org/10.1101/2024.12.09.626610

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Phenotypic diversity acts as a higher-order functional trait of populations

Functional traits are usually assigned to individuals, yet some properties exist only as differences among population members. Whether such higher-order properties predict collective performance remains unclear. Here we used the aquatic plant Spirodela polyrhiza to test whether phenotypic and genetic differences among genotypes predict non-additive population growth. We grew twelve Japanese strains alone and in all pairwise mixtures and quantified their growth dynamics. Mixtures sometimes outperformed the mean of their component monocultures, and some exceeded the better-performing monoculture, although diversity effects were negative on average. Positive effects were most strongly associated with between-strain differences in frond colour and morphology, identifying specific dimensions of phenotypic diversity as population-level predictors of synergistic growth. Genome-wide higher-level association mapping identified loci where between-strain diversity was associated with growth effects, implicating stress responses, secondary metabolism and frond morphology. Loci associated with reduced performance implicated chemical perception and self/non-self recognition, whereas those associated with positive effects reflected functional differentiation in stress responses and physiological niches. Our findings suggest that some traits generate new functions at the population level through variation among individuals, making diversity itself a higher-order functional property.

ecology↗

bioRxiv2026

Developmental cold exposure increases the ability to maintain body temperature during future cold challenges in a free-living altricial bird

Developing in suboptimal temperatures can have widespread negative effects in organisms, but early exposure to thermal challenges can also trigger increased investment in thermoregulatory capacity. Within birds, the effects of cold exposure during incubation may differ between precocial and altricial species due to differences in the ontogeny of thermoregulation. To date, most of the evidence showing that embryonic cold exposure shapes future thermoregulatory capacity comes from a few precocial species in laboratory settings. Less is known about sensitivity to low-temperature incubation in altricial species which only become fully endothermic after hatching. In this study, we tested the effects of cold temperatures during embryonic development by temporarily cooling the nest boxes of free-living tree swallows (Tachycineta bicolor) during late incubation and subsequently exposing nestlings to an acute cold challenge. Cold exposure during early development made nestlings better at maintaining their body temperatures during the acute challenge. Developmentally cold-exposed birds also had higher thyroxine (T4) levels when sampled at lower ambient temperatures, whereas control birds showed the opposite relationship. Among nestlings that failed to maintain their body temperature during the acute cold challenge, developmentally cold-exposed nestlings secreted more corticosterone during the acute cold challenge than control nestlings. Despite these changes, cold-exposed birds did not differ from controls in cold-induced metabolic rates, body mass, or bacteria killing ability. These results are, to our knowledge, the first to show that developmental cold exposure increases the capacity to maintain body temperature during future cold challenges in a songbird. These findings suggest that experiencing even a mild decline in ambient temperatures during embryonic development could prime birds to cope more effectively with harsh or variable environmental conditions later in life.

ecology↗

bioRxiv2026

Winter-run Chinook salmon juvenile recruitment and early life history response to flow in a heavily altered tailwater

Diverse life history strategies allow species to spread risk across a mosaic of habitat conditions. In Chinook Salmon (Oncorhynchus tshawytscha), particularly in the Central Valley of California, this is expressed through varied adult run timings and juvenile outmigration strategies. Anthropogenic impacts, particularly dams, disrupt these fundamental life stage transitions, often forcing managed populations to adapt to homogenized, less stochastic hydrologic regimes. This is exemplified in the Sacramento River, where the conservation of endangered Sacramento River winter run Chinook salmon requires balancing complex water operations with the ecological needs of a population confined to a short stretch of suitable habitat downstream of Shasta Reservoir. Using a 23 year dataset (2002 to 2024), we evaluated the combined effects of flow, temperature, and spawner abundance on juvenile production and life history expression in this habitat. We found that flow and spawner abundance best predicted juvenile abundance at Red Bluff Diversion Dam while temperature had considerably less support. The proportion of juveniles migrating as smolts exhibited significant density dependence that was negatively correlated to both female spawner abundance and peak flows. These results suggest focusing on temperature management alone may be insufficient. Effective management strategies should consider flow variability and habitat restoration to facilitate varying migration strategies and expand upstream rearing capacity. By addressing these physical and hydrologic constraints, managers can better support the full suite of life history strategies necessary for the resilience of winter run Chinook salmon.

ecology↗