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bioRxiv · 10.1101/2024.10.23.619533

SC10X/U: A High-density Electrode System for Non-Invasive Recording of Neural Activity of the Cervical Spinal Cord

Mehra, P.·Metzger, M.·Bista, S.·Plaitano, S.·Giglia, E.·Nash, L.·Domhnaill, E. M.·Mitchell, M.·Bede, P.·Lowery, M. M.·Muthuraman, M.·Hardiman, O.·Nasseroleslami, B.
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Abstract

ObjectiveTo design and develop a high-density (HD) electrode system that describes the position of surface electrodes for recording electrophysiological signals from the human cervical spinal cord. The system is intended to standardize experimental recordings and facilitate the subsequent analysis of evoked and spontaneous spinal cord neural activity, using high-density electrospinography (HD-ESG). MethodThe proposed system (SC10-X/U) describes the locations of up to 76 channels with a unique nomenclature, where the division of the spinal cord (SC) electrode space was inspired by the EEG 10-10 system. As proof of concept, spinal evoked potentials in response to median nerve stimulation at the wrist were recorded from 10 participants and characterized based on a 64-channel derivation from the SC10-X/U system. ResultsFollowing the design criteria, the SC10-X/U defines 76 electrode positions and its configuration. HD-ESG system was utilized to successfully record evoked spinal responses and significant N13 and P9 potential were observed in response to the stimulation. The spinal N13 potential had a latency of 13.2 +-1.1ms (mean +-SD) after stimulation. A topographic map of the N13 electro-spinal activity using the 64-channel recording system revealed an epicentre at C5-C7 dorsal-vertebral locations (ML4 - ML6 electrodes). ConclusionThe proposed SC10-X/U system will facilitate standardized recording and analysis of high-density ESG signals from the human cervical spinal cord. The system defines electrode locations to promote standardization across different individuals, studies, and clinical and research centres. The HD-ESG evoked potentials recorded using the proposed system were comparable to those observed in previous non-HD studies. The presented topographic maps conform to known neurophysiological and neuroanatomical findings. This served to validate the design and development of the electrode system and patch for future studies.

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Mehra, P., Metzger, M., Bista, S., Plaitano, S., Giglia, E., Nash, L., Domhnaill, E. M., Mitchell, M., Bede, P., Lowery, M. M., Muthuraman, M., Hardiman, O., Nasseroleslami, B.. 2024-10-23. SC10X/U: A High-density Electrode System for Non-Invasive Recording of Neural Activity of the Cervical Spinal Cord. https://doi.org/10.1101/2024.10.23.619533

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