dScaff - an automatic bioinformatics framework for scaffolding draft de novo assemblies based on reference genome data

Constantin, N. D.; Ionascu, A.; Ratiu, A. C.

doi:10.1101/2024.09.23.614313

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bioRxiv · 10.1101/2024.09.23.614313

dScaff - an automatic bioinformatics framework for scaffolding draft de novo assemblies based on reference genome data

Constantin, N. D.·Ionascu, A.·Ratiu, A. C.

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Abstract

Rapid evolution of long-read sequencing technologies requires accurate, fast and user-friendly genome assembly and scaffolding tools. In this article we present Digital Scaffolding (dScaff), a bioinformatics tool that performs scaffolding of de novo genome assemblies based on reference scaffolds or chromosomes. dScaff makes use of a series of bash and R scripts in order to create a minimal complete scaffold from a genome assembly, with future features to be implemented. Herein, we demonstrate the functionality of dScaff on a novel genome assembly pertaining to a recently sequenced local strain of Drosophila suzukii.

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Constantin, N. D., Ionascu, A., Ratiu, A. C.. 2024-09-24. dScaff - an automatic bioinformatics framework for scaffolding draft de novo assemblies based on reference genome data. https://doi.org/10.1101/2024.09.23.614313

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