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bioRxiv · 10.1101/2024.07.30.605731

Rapid neutralizing assay for circulating H5N1 influenza virus in dairy cows

Miyakawa, K.·Ota, M.·Sano, K.·Momose, F.·Okura, T.·Kishida, N.·Arita, T.·Suzuki, Y.·Shirakura, M.·Asanuma, H.·Watanabe, S.·Ryo, A.·Hasegawa, H.
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Abstract

A rapid and safe neutralization assay is required for emerging highly pathogenic avian influenza viruses, including the H5N1 subtype, which was recently found in cows. Herein, we report a novel neutralization assay using HiBiT-tagged virus-like particles (hiVLPs). Our hiVLP-based neutralization test demonstrated a higher quantitative value and shorter assay time than conventional methods. We used this assay to evaluate whether the neutralizing antibodies induced by the candidate vaccine virus (NIID-002) were cross-reactive with cow-derived H5N1. Our results suggest that the circulating H5N1 virus in cows shares antigenic characteristics with NIID-002, providing significant implications for the development and preparation of vaccines.

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Miyakawa, K., Ota, M., Sano, K., Momose, F., Okura, T., Kishida, N., Arita, T., Suzuki, Y., Shirakura, M., Asanuma, H., Watanabe, S., Ryo, A., Hasegawa, H.. 2024-07-30. Rapid neutralizing assay for circulating H5N1 influenza virus in dairy cows. https://doi.org/10.1101/2024.07.30.605731

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