Molecular signatures of cortical expansion in the human fetal brain

Ball, G.; Oldham, S.; Kyriakopoulou, V.; Williams, L. Z. J.; Karolis, V. R.; Price, A.; Hutter, J.; Seal, M. L.; Alexander-Bloch, A.; Hajnal, J. V.; Edwards, A. D.; Robinson, E. C.; Seidlitz, J.

doi:10.1101/2024.02.13.580198

bioRxiv · 10.1101/2024.02.13.580198

Molecular signatures of cortical expansion in the human fetal brain

Ball, G.·Oldham, S.·Kyriakopoulou, V.·Williams, L. Z. J.·Karolis, V. R.·Price, A.·Hutter, J.·Seal, M. L.·Alexander-Bloch, A.·Hajnal, J. V.·Edwards, A. D.·Robinson, E. C.·Seidlitz, J.

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Abstract

The third trimester of human gestation is characterised by rapid increases in brain volume and cortical surface area. A growing catalogue of cells in the prenatal brain has revealed remarkable molecular diversity across cortical areas.1,2 Despite this, little is known about how this translates into the patterns of differential cortical expansion observed in humans during the latter stages of gestation. Here we present a new resource, Brain, to facilitate knowledge translation between molecular and anatomical descriptions of the prenatal developing brain. Built using generative artificial intelligence, Brain is a three-dimensional cellular-resolution digital atlas combining publicly-available serial sections of the postmortem human brain at 21 weeks gestation3 with bulk tissue microarray data, sampled across 29 cortical regions and 5 transient tissue zones.4 Using Brain, we evaluate the molecular signatures of preferentially-expanded cortical regions during human gestation, quantified in utero using magnetic resonance imaging (MRI). We find that differences in the rates of expansion across cortical areas during gestation respect anatomical and evolutionary boundaries between cortical types5 and are founded upon extended periods of upper-layer cortical neuron migration that continue beyond mid-gestation. We identify a set of genes that are upregulated from mid-gestation and highly expressed in rapidly expanding neocortex, which are implicated in genetic disorders with cognitive sequelae. Our findings demonstrate a spatial coupling between areal differences in the timing of neurogenesis and rates of expansion across the neocortical sheet during the prenatal epoch. The Brain atlas is available from: https://garedaba.github.io/micro-brain/ and provides a new tool to comprehensively map early brain development across domains, model systems and resolution scales.

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Ball, G., Oldham, S., Kyriakopoulou, V., Williams, L. Z. J., Karolis, V. R., Price, A., Hutter, J., Seal, M. L., Alexander-Bloch, A., Hajnal, J. V., Edwards, A. D., Robinson, E. C., Seidlitz, J.. 2024-02-13. Molecular signatures of cortical expansion in the human fetal brain. https://doi.org/10.1101/2024.02.13.580198

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