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bioRxiv · 10.1101/2023.11.11.566669

Intestinal cDC1s provide IL-12 dependent and independent functions required for CD4+ T cell mediated resistance to Cryptosporidium

Abstract

Cryptosporidium is an enteric pathogen that is a prominent cause of diarrheal disease. Control of this infection requires CD4+ T cells, though the processes that lead to T cell-mediated resistance have been difficult to assess. Here, Cryptosporidium parasites that express MHCII-restricted model antigens were generated to dissect the early events that influence CD4+ T cell priming and effector function. These studies highlight that parasite-specific CD4+ T cells are primed in the draining mesenteric lymph node (mesLN) and differentiate into Th1 cells in the gut, where they mediate IFN-{gamma}-dependent control of the infection. Although type 1 conventional dendritic cells (cDC1s) were not required for initial priming of CD4+ T cells, cDC1s were required for CD4+ T cell expansion and gut homing. cDC1s were also a major source of IL-12 that was not required for priming but promoted full differentiation of CD4+ T cells and local production of IFN-{gamma}. Together, these studies reveal distinct roles for cDC1s in shaping CD4+ T cell responses to enteric infection: first to drive early expansion in the mesLN and second to drive effector responses in the gut. SummaryCryptosporidium parasites that express model antigens were generated to dissect how parasite-specific CD4+ T cells are primed and mediate effector functions required to control this enteric pathogen. cDC1s produced IL-12p40 and were required for early expansion and gut homing of CD4+ T cells. However, IL-12p40 was only required for the development of Th1 CD4+ T cell effector function in the gut.

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BibTeXRIS

Cohn, I. S., Wallbank, B. A., Haskins, B. E., O'Dea, K. M., Pardy, R. D., Shaw, S., Merolle, M. I., Gullicksrud, J., Christian, D. A., Striepen, B., Hunter, C. A.. 2023-11-13. Intestinal cDC1s provide IL-12 dependent and independent functions required for CD4+ T cell mediated resistance to Cryptosporidium. https://doi.org/10.1101/2023.11.11.566669

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