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bioRxiv · 10.1101/2023.08.10.552756

One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes

Ye, Z.·Sabatier, P.·Martin-Gonzalez, J.·Eguchi, A.·Bekker-Jensen, D. B.·Bache, N.·Olsen, J. V.
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Abstract

We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from [~]1000 cells and [~] 6,000 proteins in a single mouse zygote with a throughput of 40 samples-per-day. This easy-to-use method expands capabilities of proteomics research by enabling greater depth, scalability and throughput covering low to high input samples.

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Ye, Z., Sabatier, P., Martin-Gonzalez, J., Eguchi, A., Bekker-Jensen, D. B., Bache, N., Olsen, J. V.. 2023-08-11. One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes. https://doi.org/10.1101/2023.08.10.552756

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