HIC1 interacts with FOXP3 multi-protein complex: a novel mechanism to regulate human regulatory T cell differentiation and function

Andrabi, S. B. A.; Batkulwar, K.; Bhosale, S. D.; Moulder, R.; Khan, M. H.; Buchacher, T.; Khan, M. M.; Rasool, O.; Kalim, U. U.; Lahesmaa, R.

doi:10.1101/2023.05.15.540505

bioRxiv · 10.1101/2023.05.15.540505

HIC1 interacts with FOXP3 multi-protein complex: a novel mechanism to regulate human regulatory T cell differentiation and function

Andrabi, S. B. A.·Batkulwar, K.·Bhosale, S. D.·Moulder, R.·Khan, M. H.·Buchacher, T.·Khan, M. M.·Rasool, O.·Kalim, U. U.·Lahesmaa, R.

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Abstract

Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates in a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides a significant contribution to the function and development of regulatory T (Treg) cells. However, the mechanism by which it regulates these processes was not apparent. To address this question, we used affinity-purification mass spectrometry to characterize the HIC1 Interactome in human Treg cells. Altogether 61 high-confidence interactors were identified, including IKZF3, which is a key transcription factor in the development of Treg cells. The biological processes associated with these interacting proteins include protein transport, mRNA processing, non-coding (ncRNA) transcription and RNA metabolism. The results revealed that HIC1 is part of a FOXP3-RUNX1-CBFB protein complex that regulates Treg signature genes thus improving our understanding of HIC1 function during early Treg cell differentiation. HighlightsO_LISystematic characterization of HIC1 interactome in regulatory T cells by Affinity Purification-Mass Spectrometry C_LIO_LIHIC1 binds to the RUNX1 promoter and regulates its expression C_LIO_LIHIC1-a part of FOXP3-RUNX1-CBFB transcriptional complex C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=37 SRC="FIGDIR/small/540505v1_ufig1.gif" ALT="Figure 1"> View larger version (14K): org.highwire.dtl.DTLVardef@1fcb119org.highwire.dtl.DTLVardef@cf1397org.highwire.dtl.DTLVardef@10f512forg.highwire.dtl.DTLVardef@9ae94e_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Andrabi, S. B. A., Batkulwar, K., Bhosale, S. D., Moulder, R., Khan, M. H., Buchacher, T., Khan, M. M., Rasool, O., Kalim, U. U., Lahesmaa, R.. 2023-05-15. HIC1 interacts with FOXP3 multi-protein complex: a novel mechanism to regulate human regulatory T cell differentiation and function. https://doi.org/10.1101/2023.05.15.540505

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