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bioRxiv · 10.1101/2023.02.02.526918

Different roles of dFOXO and HSF in spatio-temporal dynamics of stress-inducible Hsp70 in lgl-yorkie & lgl-Ras driven epithelial tumours in Drosophila

Singh, G.·Lakhotia, S. C.
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Abstract

Oncogenic stress responses are critical modulators of tumour cell adaptation to hostile microenvironments and clonal dynamics. The evolutionarily conserved Hsp70 chaperone promotes tumour cell survival by suppressing apoptosis and facilitating metastatic progression. However, the temporal order and regulation of its expression in neoplastic tumours remains ill-defined. Here, we show a non-canonical regulation of Hsp70 expression during neoplastic transformation in the Drosophila wing epithelium. The malignant lgl ykiOE MARCM clones exhibited a delayed and progressive induction of Hsp70, tightly correlated with clonal expansion and tissue invasion. Loss of Hsp70 function in developing lgl ykiOE clones suppressed tumour growth in both larval wing discs and allograft assays, underscoring its significant role in tumour fitness and growth. We identify a non-canonical pathway where ROS-activated JNK signalling drives FOXO-dependent induction of Hsp70, bypassing HSF/Hif1, to promote tumour growth and invasion. Genetic disruption of Nox or JNK abrogated Hsp70 induction and curtailed tumour expansion. Stress adaptation and malignancy of developing lgl ykiOE epithelial tumour thus require Hsp70 as a redox-responsive, temporally regulated effector of the JNK-FOXO axis.

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Singh, G., Lakhotia, S. C.. 2023-02-03. Different roles of dFOXO and HSF in spatio-temporal dynamics of stress-inducible Hsp70 in lgl-yorkie & lgl-Ras driven epithelial tumours in Drosophila. https://doi.org/10.1101/2023.02.02.526918

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