Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2023.01.14.523685

Relationship between the ACTN3, ACE, AGT, BDKRB2 and IL6 genes and the intake of creatine HCl, whey protein and glutamine, with changes in strength and fat percentage, before an undulating strength program in lower limbs in athletes from Valle del Cauca. Colombia

Gonzalez Estrada, G. D.·Paz, E.·Sanclemente, F.
View original source ↗Export metadata ↓

Abstract

Changes in power, strength and muscle mass gain were measured with a group of university athletes (n=11), separating them into two groups, one with supplementation and the other without. supplementation, to determine if the intake of sports supplements had an influence or not on individuals with similar genotypic profiles, or the results of the tests only depended on the predisposition to strength and muscle gain of the ACE, ACTN3, AGT, IL6 and BDKRB2 genes. Genotyping was performed based on PCR, RFLP and polyacrylamide electrophoresis tests. The supplemented group ingested whey protein, creatine HCl, and glutamine. All individuals underwent undulating strength training for four months and jump power tests (SJ, CMJ, and ABA), 1RM, and bioimpedance were performed at three different times. Changes were obtained in all the athletes, but the group that obtained the greatest gains in all the tests, except the CMJ jump, was the supplemented group and also had a genotypic profile that registered the lowest TGS. In conclusion, we observed significant improvements in individuals with lower TGS and taking sports supplements, surpassing the group that did not take supplements, but had a greater genetic predisposition in strength activities.

Explore related subjects

geneticsPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Gonzalez Estrada, G. D., Paz, E., Sanclemente, F.. 2023-01-14. Relationship between the ACTN3, ACE, AGT, BDKRB2 and IL6 genes and the intake of creatine HCl, whey protein and glutamine, with changes in strength and fat percentage, before an undulating strength program in lower limbs in athletes from Valle del Cauca. Colombia. https://doi.org/10.1101/2023.01.14.523685

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Host control and regulation of human anellovirus persistence

Anelloviruses are the most abundant and diverse eukaryotic viruses known to infect humans and can persist throughout our lifetimes. Despite their ubiquity, the host factors governing their physiologic distribution and abundance remain poorly understood. Here, we construct a viral pangenome of 2,023 strains to quantify anellovirus DNA in >1.1 million participants spanning three genetic biobanks. Genome-wide association studies identify 121 loci linked to viral persistence, predominantly via immune regulation by NF-kB signaling. Further, we associate variable anellovirus detection with conditions characterized by immune dysfunction, including lymphoid malignancies. By screening 3.2 petabases of human single-cell sequencing data, we identify myeloid progenitors in the bone marrow as a prominent site of anellovirus transcription in vivo. Finally, Perturb-seq of anellovirus proteins reveals that anellovirus ORF2, particularly from Alphatorquevirus, attenuates TNF-a-induced NF-kB signaling, connecting host genetic variation, cellular context, and viral immunomodulation to characterize the persistence of the eukaryotic human virome's most abundant component.

genetics↗
bioRxiv2026

SARDINE unlocks EWAS summary statistics for large-scale epigenetic biomarker analysis

Epigenetic clocks are increasingly used to study biological aging, yet their interpretation and utility remain uncertain. Meanwhile, the growing wealth of epigenome-wide association study (EWAS) summary statistics remains largely untapped for evaluating these biomarkers. Here, we introduce SARDINE, a statistical framework that estimates epigenetic clock-phenotype associations using EWAS summary statistics, clock weights, and reference methylation covariance, without individual-level data. SARDINE accurately reproduced individual-level association estimates across 38 epigenetic biomarkers and four phenotypes in 15,677 individuals. Applying SARDINE to diverse EWAS datasets revealed substantial heterogeneity in biomarker associations with aging, diseases, and lifestyle factors. By transforming CpG-level EWAS results into interpretable biomarker-level associations, SARDINE unlocks a largely underutilized data resource and establishes a scalable paradigm for systematic biomarker evaluation and discovery in aging research.

genetics↗
bioRxiv2026

Deep transcriptomic analysis of molecular mediators of complex traits in heterogeneous stock (HS) rats

Rats are a powerful model organism for genome-wide association studies (GWAS) of physiological and behavioral traits. Heterogeneous Stock (HS) rats, which were created by intercrossing 8 inbred rat strains, are heavily used for this purpose because of their genetic diversity and accumulated recombinations due to over 100 generations of outbreeding, which improves mapping precision. However, functional genomics resources for post-GWAS interpretation of genomic findings have been limited. We processed 3,160 RNA-seq samples, 1,932 of which were previously unpublished, from 13 tissues as part of RatGTEx. We used Pantry to quantify six modalities of transcriptome regulation (expression, isoform ratio, intron excision ratio, alternative transcription start site (TSS), alternative poly(A), and RNA stability) in each sample, which allowed us to identify 144,876 independent quantitative trait loci (QTL) for 22,599 genes. We performed a transcriptome-wide association study (TWAS) with 19,796 unique genes across tissues and transcriptional modalities and 308 traits collected from 14 studies using FUSION, which identified 7,473 significant gene-trait associations. This work presents a significant expansion of the RatGTEx resource in terms of samples, modes of transcriptome regulation, and genetic analyses. Our findings provide molecular evidence for the involvement of specific genes in mediating GWAS signals through a range of transcriptome regulatory mechanisms. These gene-to-phenotype relationships can be easily applied to genetic analyses in humans and other species. Further experiments can use this evidence to confirm these molecular mediators and target them for the treatment of behavioral and physiological traits and disorders. To facilitate use of these results, we have updated results on RatGTEx.org and provided a new interactive TWAS web portal (twas.ratgtex.org).

genetics↗