Gonzalez Estrada, G. D. | Search bioRxiv

bioRxiv2023

Relationship between the ACTN3, ACE, AGT, BDKRB2 and IL6 genes and the intake of creatine HCl, whey protein and glutamine, with changes in strength and fat percentage, before an undulating strength program in lower limbs in athletes from Valle del Cauca. Colombia

Changes in power, strength and muscle mass gain were measured with a group of university athletes (n=11), separating them into two groups, one with supplementation and the other without. supplementation, to determine if the intake of sports supplements had an influence or not on individuals with similar genotypic profiles, or the results of the tests only depended on the predisposition to strength and muscle gain of the ACE, ACTN3, AGT, IL6 and BDKRB2 genes. Genotyping was performed based on PCR, RFLP and polyacrylamide electrophoresis tests. The supplemented group ingested whey protein, creatine HCl, and glutamine. All individuals underwent undulating strength training for four months and jump power tests (SJ, CMJ, and ABA), 1RM, and bioimpedance were performed at three different times. Changes were obtained in all the athletes, but the group that obtained the greatest gains in all the tests, except the CMJ jump, was the supplemented group and also had a genotypic profile that registered the lowest TGS. In conclusion, we observed significant improvements in individuals with lower TGS and taking sports supplements, surpassing the group that did not take supplements, but had a greater genetic predisposition in strength activities.

genetics↗

bioRxiv2022

Association of variants in ACE, ACTN3, AGT, IL6 and BDKRB2 genes with athlete status and playing position in Colombian amateur rugby athletes

Genetic polymorphisms are involved in different metabolic pathways that are manifested at the physiological level and have been associated with specific phenotypes in sport from anthropometric and functional characterizations that pose conditional and physiological demands for the rugby athlete. The identification of this type of polymorphisms in athletes represents a resource that contributes significantly to the processes of training, selection and sports orientation. The purpose of this study was to describe type and frequencies of allelic and genotypic variants in ACTN3, ACE, AGT, BDRKB2 and IL6 genes in sub elite rugby athletes in Colombia. Additionally, the polymorphisms found were compared with a control population, as well as contrasted according to playing position backs and forwards. In this research, 47 individuals from the Vallecaucana rugby league and 67 from a control group (non-athletes) were sampled. All were analyzed for polymorphisms in the ACE, AGT, ACTN3, IL6 and BDKRB2 genes, using the PCR RFLPs technique. The significance of the differences between the experimental and control groups was tested by the X2 test (p <0.05). In rugby athletes we found a higher frequency of allele D (0.883) ACE gene, allele R (0.63) ACTN3 gene, allele G (0.819) IL6 gene, all associated with strength and power sports. There are significant genotypic differences between athletes and the control population in all the genes analyzed and significant allelic differences in the ACE, ACTN3, BDRKB2 and IL6 genes. When comparing the playing positions (backs vs. forwards), significant genotypic differences were observed in the ACTN3, BDRKB2, AGT and IL6 genes. At the allelic level, the R and X alleles of the ACTN3 gene and the I allele of the ACE gene show significant differences. In conclusion, in the polymorphisms analyzed, an association with strength sports, explosive strength and rugby is observed. Significant genotypic and allelic differences were also recorded between the backs and forwards positions, as well as significant differences in the allelic and genotypic structure between the group of athletes and the control population.

genetics↗