3-D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System (MANTIS)

Scholaert, M.; Houmadi, R.; Martin, J.; Serhan, N.; Tauber, M.; Braun, E.; Basso, L.; Merle, E.; Descargues, P.; Viguier, M.; Lesort, C.; Chaput, B.; Kanitakis, J.; Jullien, D.; Bulai-Livideanu, C.; Lamant, L.; Pages, E.; Gaudenzio, N.

doi:10.1101/2023.01.13.523748

bioRxiv · 10.1101/2023.01.13.523748

3-D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System (MANTIS)

Scholaert, M.·Houmadi, R.·Martin, J.·Serhan, N.·Tauber, M.·Braun, E.·Basso, L.·Merle, E.·Descargues, P.·Viguier, M.·Lesort, C.·Chaput, B.·Kanitakis, J.·Jullien, D.·Bulai-Livideanu, C.·Lamant, L.·Pages, E.·Gaudenzio, N.

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Abstract

Routine clinical assays, such as conventional immunohistochemistry, often fail to resolve the regional heterogeneity of complex inflammatory skin conditions. Here we introduce MANTIS (Multiplexed Annotated Tissue Imaging System), a flexible analytic pipeline compatible with routine practice, specifically-designed for spatially-resolved immune phenotyping of the skin in experimental or clinical samples. Based on phenotype attribution matrices coupled to -shape algorithms, MANTIS projects a representative digital immune landscape, while enabling automated detection of major inflammatory clusters and concomitant single-cell data quantification of biomarkers. We observed that severe pathological lesions from systemic lupus erythematosus, Kawasaki syndrome or COVID-19-associated skin manifestations share common quantitative immune features, while displaying a non-random distribution of cells with the formation of disease-specific dermal immune structures. Given its accuracy and flexibility, MANTIS is designed to solve the spatial organization of complex immune environments to better apprehend the pathophysiology of skin manifestations.

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Scholaert, M., Houmadi, R., Martin, J., Serhan, N., Tauber, M., Braun, E., Basso, L., Merle, E., Descargues, P., Viguier, M., Lesort, C., Chaput, B., Kanitakis, J., Jullien, D., Bulai-Livideanu, C., Lamant, L., Pages, E., Gaudenzio, N.. 2023-01-17. 3-D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System (MANTIS). https://doi.org/10.1101/2023.01.13.523748

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