Microbe-induced plant drought tolerance by ABA-mediated root morphogenesis and epigenetic reprogramming of gene expression

Alwutayd, K. M.; Rawat, A. R.; Sheikh, A.; Almeida-Trapp, M.; Veluchamy, A.; Jalal, R.; Karampelias, M.; Froehlich, K.; Alzaed, W.; Tabassum, N.; Schley, T. R.; Schaeffner, A. R.; Daur, I.; Saad, M. M.; Hirt, H.

doi:10.1101/2023.01.03.522604

bioRxiv · 10.1101/2023.01.03.522604

Microbe-induced plant drought tolerance by ABA-mediated root morphogenesis and epigenetic reprogramming of gene expression

Alwutayd, K. M.·Rawat, A. R.·Sheikh, A.·Almeida-Trapp, M.·Veluchamy, A.·Jalal, R.·Karampelias, M.·Froehlich, K.·Alzaed, W.·Tabassum, N.·Schley, T. R.·Schaeffner, A. R.·Daur, I.·Saad, M. M.·Hirt, H.

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Abstract

The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis sp. SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesis and gene expression is mediated via the plant abscisic acid (ABA) pathway. Moreover, we demonstrate that SA190 primes the promoters of target genes in an epigenetic manner which is ABA-dependent. Application of the SA190 priming technology on crops is demonstrated for alfalfa in field trials, showing enhanced performance under desert agriculture conditions. In summary, a single beneficial root bacterial strain can help to perform agriculture under drought and water limiting conditions. Synopsis O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=161 SRC="FIGDIR/small/522604v1_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@b0bd17org.highwire.dtl.DTLVardef@14e3262org.highwire.dtl.DTLVardef@cdd103org.highwire.dtl.DTLVardef@5526a0_HPS_FORMAT_FIGEXP M_FIG C_FIG O_LIBeneficial root endophyte Pseudomonas argentinensis sp. SA190 confers drought tolerance in plants C_LIO_LISA190 modulates the expression of genes under drought stress in an ABA-dependent manner C_LIO_LISA190 primes genes via H3K4me3 histone mark enrichment C_LIO_LISA190 alters host plant physiology by improving the plant water status C_LIO_LISA190 enhances crop performance in open field conditions with limited irrigation C_LI

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Alwutayd, K. M., Rawat, A. R., Sheikh, A., Almeida-Trapp, M., Veluchamy, A., Jalal, R., Karampelias, M., Froehlich, K., Alzaed, W., Tabassum, N., Schley, T. R., Schaeffner, A. R., Daur, I., Saad, M. M., Hirt, H.. 2023-01-07. Microbe-induced plant drought tolerance by ABA-mediated root morphogenesis and epigenetic reprogramming of gene expression. https://doi.org/10.1101/2023.01.03.522604

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