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bioRxiv · 10.1101/2022.11.29.518428

Dynamic Data Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment

Heil, L. R.·Remes, P. M.·Canterbury, J. D.·Yip, P.·Barshop, W. D.·Wu, C. C.·MacCoss, M. J.
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Abstract

We report a data independent acquisition (DIA) strategy that dynamically adjusts the tandem mass spectrometry (MS/MS) windows during the chromatographic separation. The method focuses MS/MS acquisition on the most relevant mass range at each point in time - improving the quantitative sensitivity by increasing the time spent on each DIA window. We demonstrate an improved lower limit of quantification, on average, without sacrificing the number of peptides detected.

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Heil, L. R., Remes, P. M., Canterbury, J. D., Yip, P., Barshop, W. D., Wu, C. C., MacCoss, M. J.. 2022-12-02. Dynamic Data Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment. https://doi.org/10.1101/2022.11.29.518428

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