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bioRxiv · 10.1101/2022.10.28.514297

Hormone-induced enhancer assembly requires an optimal level of hormone receptor multivalent interactions

Abstract

Transcription factors (TFs) activate enhancers to drive cell-specific gene programs in response to signals, but our understanding of enhancer assembly during signaling events is incomplete. Here, we show that Androgen Receptor (AR), a steroid hormone-regulated transcription factor, forms condensates through multivalent interactions in response to androgen signaling to orchestrate enhancer assembly. We demonstrate that the intrinsically disordered N-terminal domain (NTD) of AR drives 1,6-Hexanediol-sensitive condensate formation and that NTD deletion or aromatic residue mutation reduces AR self-association and abolishes AR transcriptional activity. AR NTD can be substituted by intrinsically disordered regions (IDRs) from selective proteins for AR condensation capacity and transactivation function. Surprisingly, strengthened AR condensation capacity caused by extending the polyQ tract within AR NTD also leads to impaired transcriptional activity without affecting AR binding on enhancers. Furthermore, either NTD deletion or polyQ extension reduces heterotypic multivalent interactions between AR and other enhancer components. These results suggest the importance of an optimal level of AR condensation in mediating AR-AR homotypic and AR-cofactor heterotypic interactions to regulate enhancer assembly in response to signals. Our study supports the notion that alteration of the fine-tuned multivalent IDR-IDR interactions might underlie AR-related human pathologies, thereby providing novel molecular insights for potential therapeutic strategies to treat prostate cancer and other AR-involved diseases by targeting AR multivalent interactions.

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BibTeXRIS

Chen, L., Zhang, Z., Han, Q., Rodrigues, L., Zboril, E., Adhikari, R., Li, X., Ko, S.-H., Xue, P., Smith, E., Xu, K., Wang, Q., Huang, T. H.-M., Chong, S., Liu, Z.. 2022-10-30. Hormone-induced enhancer assembly requires an optimal level of hormone receptor multivalent interactions. https://doi.org/10.1101/2022.10.28.514297

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