RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions

Wells, J.; SRIVASTAVA, A.; Maser, R. S.; Kesharwani, R.; Sarsani, V. K.; Daigle, S. L.; Lynch, R. P.; Munger, H. J.; Doty, R.; Saxl, R. L.; Bult, C. J.

doi:10.1101/2022.08.22.504764

bioRxiv · 10.1101/2022.08.22.504764

RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions

Wells, J.·SRIVASTAVA, A.·Maser, R. S.·Kesharwani, R.·Sarsani, V. K.·Daigle, S. L.·Lynch, R. P.·Munger, H. J.·Doty, R.·Saxl, R. L.·Bult, C. J.

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Abstract

MicroRNAs (miRNAs) are crucial post-transcriptional regulators of gene expression in species ranging from plants to mammals. Unraveling the complex networks of miRNA-mediated regulation of gene expression requires the ability to identify the mRNA targets of individual miRNAs in native cellular contexts. Here we describe RIP-SIR, an unbiased genome-wide method for identifying interactions between endogenous miRNAs and their targets in almost any tissue or cell type.

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Wells, J., SRIVASTAVA, A., Maser, R. S., Kesharwani, R., Sarsani, V. K., Daigle, S. L., Lynch, R. P., Munger, H. J., Doty, R., Saxl, R. L., Bult, C. J.. 2022-08-22. RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions. https://doi.org/10.1101/2022.08.22.504764

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