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bioRxiv · 10.1101/2022.08.01.502378

Global Profiling of Histone Modifications in Plasmodium falciparum using CUT&RUN.

Morillo, R. C.·Harris, C. T.·Kennedy, K.·Kafsack, B. F. C.
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Abstract

We recently adapted a CUT&RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium. Using the step-by-step protocol described below, we were able to generate high quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIPseq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&RUN profiling is highly reproducible and closely recapitulates previously published ChIPseq-based abundance profiles of histone marks. Finally, we show that CUT&RUN requires substantially lower sequencing coverage for accurate profiling compared to ChIPseq.

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Morillo, R. C., Harris, C. T., Kennedy, K., Kafsack, B. F. C.. 2022-08-03. Global Profiling of Histone Modifications in Plasmodium falciparum using CUT&RUN.. https://doi.org/10.1101/2022.08.01.502378

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