A Non-Canonical Function of Arabidopsis ERECTA Proteins in Gibberellin Signaling

Sarnowska, E.; Kubala, S.; Cwiek, P.; Sacharowski, S.; Oksinska, P.; Steciuk, J.; Zaborowska, M.; Szurmak, J. M.; Dubianski, R.; Maassen, A.; Stachowiak, M.; Huettel, B.; Ciesla, M.; Nowicka, K.; Rolicka, A. T.; Alseekh, S.; Bucior, E.; Franzen, R.; Klepacz, A.; Domagalska, M. A.; Amar, S.; Fernie, A. R.; Davis, S. J.; Sarnowski, T. J.

doi:10.1101/2021.12.02.470991

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bioRxiv · 10.1101/2021.12.02.470991

A Non-Canonical Function of Arabidopsis ERECTA Proteins in Gibberellin Signaling

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Abstract

The Arabidopsis ERECTA family (ERf) of leucine-rich repeat receptor-like kinases (LRR-RLKs), comprising ERECTA (ER), ERECTA-LIKE 1 (ERL1) and ERECTA-LIKE 2 (ERL2), control epidermal patterning, inflorescence architecture, stomata development, and hormonal signaling. Here we show that the er/erl1/erl2 triple mutant exhibits impaired gibberellin (GA) biosynthesis and perception alongside broad transcriptional changes. ERf proteins interact in the nucleus, via kinase domains, with the SWI3B subunit of the SWI/SNF chromatin remodeling complex (CRCs). The er/erl1/erl2 triple mutant exhibits reduced SWI3B protein level and affected nucleosomal chromatin structure. The ER kinase phosphorylates SWI3B in vitro, and the inactivation of all ERf proteins leads to the decreased phosphorylation of SWI3B protein in vivo. Correlation between DELLA overaccumulation and SWI3B proteasomal degradation together with the physical interaction of SWI3B with DELLA proteins explain the lack of RGA accumulation in the GA- and SWI3B-deficient erf mutant plants. Co-localization of ER and SWI3B on GID1 (GIBBERELLIN INSENSITIVE DWARF 1) DELLA target gene promoter regions and abolished SWI3B binding to GID1 promoters in er/erl1/erl2 plants supports the conclusion that ERf-SWI/SNF CRC interaction is important for transcriptional control of GA receptors. Thus, the involvement of ERf proteins in transcriptional control of gene expression, and observed similar features for human HER2 (Epidermal Growth Family Receptor-member), indicate an exciting target for further studies of evolutionarily conserved non-canonical functions of eukaryotic membrane receptors. ONE SENTENCE SUMMARYERECTA leucine-rich receptor-like kinase and SWI3B subunit of SWI/SNF chromatin remodeling complex cooperate in direct transcriptional control of GID1 genes in Arabidopsis.

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Sarnowska, E., Kubala, S., Cwiek, P., Sacharowski, S., Oksinska, P., Steciuk, J., Zaborowska, M., Szurmak, J. M., Dubianski, R., Maassen, A., Stachowiak, M., Huettel, B., Ciesla, M., Nowicka, K., Rolicka, A. T., Alseekh, S., Bucior, E., Franzen, R., Klepacz, A., Domagalska, M. A., Amar, S., Fernie, A. R., Davis, S. J., Sarnowski, T. J.. 2021-12-03. A Non-Canonical Function of Arabidopsis ERECTA Proteins in Gibberellin Signaling. https://doi.org/10.1101/2021.12.02.470991

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