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bioRxiv · 10.1101/2021.11.26.470138

Noise in a metabolic pathway leads to persister formation in Mycobacterium tuberculosis

Abstract

Tuberculosis is difficult to treat due to dormant cells in hypoxic granulomas, and stochastically-formed persisters tolerant of antibiotics. Bactericidal antibiotics kill by corrupting their energy-dependent targets. We reasoned that noise in the expression of an energy-generating component will produce rare persister cells. In sorted low ATP M. tuberculosis grown on acetate there is considerable cell-to-cell variation in the level of mRNA coding for AckA, the acetate kinase. Quenching the noise by overexpressing ackA sharply decreases persisters, showing that it acts as the main persister gene under these conditions. This demonstrates that a low energy mechanism is responsible for the formation of M. tuberculosis persisters and suggests that the mechanism of their antibiotic tolerance is similar to that of dormant cells in a granuloma. Entrance into a low energy state driven by stochastic variation in expression of energy-producing enzymes is likely a general mechanism by which bacteria produce persisters.

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BibTeXRIS

Quigley, J., Lewis, K.. 2021-11-26. Noise in a metabolic pathway leads to persister formation in Mycobacterium tuberculosis. https://doi.org/10.1101/2021.11.26.470138

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