A novel and highly divergent Canine Distemper Virus lineage causing distemper in ferrets in Australia

George, A. M.; Wille, M.; Wang, J.; Anderson, K.; Cohen, S.; Moselen, J.; Lee, L. Y. Y.; Suen, W. W.; Bingham, J.; Dalziel, A. E.; Hurt, A. C.; Williams, D. T.; Deng, Y.-M.; Barr, I. G.

doi:10.1101/2021.11.03.467217

bioRxiv · 10.1101/2021.11.03.467217

A novel and highly divergent Canine Distemper Virus lineage causing distemper in ferrets in Australia

George, A. M.·Wille, M.·Wang, J.·Anderson, K.·Cohen, S.·Moselen, J.·Lee, L. Y. Y.·Suen, W. W.·Bingham, J.·Dalziel, A. E.·Hurt, A. C.·Williams, D. T.·Deng, Y.-M.·Barr, I. G.

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Abstract

Canine distemper virus (CDV) is a highly contagious systemic viral disease of dogs, that regularly spills-over into other animal species. Despite widespread vaccination, CDV remains endemic in many parts of the world. In this study we report an outbreak of distemper in ferrets in two independent research facilities in Australia. We found that disease severity varied, although most animals had mild to moderate disease signs. Histopathology results of animals with severe disease presented the typical profile of distemper pathology with multi-system virus replication. Through the development of a discriminatory PCR paired with full genome sequencing we revealed that the outbreak at both facilities was caused by a single, novel lineage of CDV. This lineage was highly divergent across the H gene, F signal peptide and full genome and had less than 93% similarity across the H gene to other described lineages, including the vaccine strain. Molecular analysis indicates that this strain belongs to a distinct lineage that diverged from other clades approximately 140 to 400 years ago, and appears to be unique to Australia. Given the differences in key viral proteins of this novel CDV strain, a review of the efficacy of the CDV vaccines currently in use in Australia is warranted to ensure maximum protection of dogs and other vulnerable species. In addition, enhanced surveillance to determine the prevalence of CDV in ferrets, dogs and other at-risk species in Australia would be useful to better understand the diversity of CDV in Australia. ImportanceCanine distemper virus (CDV) is highly contagious and while dogs are the main reservoir, it may spill over into a number of other animal species. In this study we report an outbreak of distemper in ferrets in two research facilities in Australia. Outcomes of pathology and histopathology suggest ferrets have widespread multi-systemic infection, consistent with previously reported distemper infections in ferrets and dogs. Critically, through sequencing and phylogenetic analysis, we revealed that the outbreak at both facilities was caused by a single, novel and highly divergent lineage of CDV. This virus had less than 93% nucleotide similarity to other described lineages and the vaccine strain. This manuscript adds considerably to the epidemiology, ecology and evolution of this virus, and is one of few reports of distemper in Australia in the literature.

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George, A. M., Wille, M., Wang, J., Anderson, K., Cohen, S., Moselen, J., Lee, L. Y. Y., Suen, W. W., Bingham, J., Dalziel, A. E., Hurt, A. C., Williams, D. T., Deng, Y.-M., Barr, I. G.. 2021-11-04. A novel and highly divergent Canine Distemper Virus lineage causing distemper in ferrets in Australia. https://doi.org/10.1101/2021.11.03.467217

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