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bioRxiv · 10.1101/2021.10.19.464928

Dynamic proton-dependent motors power Type IX secretion and gliding adhesin movement in Flavobacterium

Abstract

Motile bacteria usually rely on external apparatus like flagella for swimming or pili for twitching. By contrast, gliding bacteria do not rely on obvious surface appendages to move on solid surfaces. Flavobacterium johnsoniae and other bacteria in the Bacteroidetes phylum use adhesins whose movement on the cell surface supports motility. In F. johnsoniae, secretion and helicoidal motion of the main adhesin SprB are intimately linked and depend on the type IX secretion system (T9SS). Both processes necessitate the proton motive force (PMF), which is thought to fuel a molecular motor that comprises the GldL and GldM cytoplasmic membrane proteins. Here we show that F. johnsoniae gliding motility is powered by the pH gradient component of the PMF. We further delineate the interaction network between the GldLM transmembrane helices (TMH) and show that conserved glutamate residues in GldL TMH are essential for gliding motility, although having distinct roles in SprB secretion and motion. We then demonstrate that the PMF and GldL trigger conformational changes in the GldM periplasmic domain. We finally show that multiple GldLM complexes are distributed in the membrane suggesting that a network of motors may be present to move SprB along a helical path on the cell surface. Altogether, our results provide evidence that GldL and GldM assemble dynamic membrane channels that use the proton gradient to power both T9SS-dependent secretion of SprB and its motion at the cell surface.

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BibTeXRIS

Vincent, M. S., Comas Hervada, C., Sebban-Kreuzer, C., Le Guenno, H., Chabalier, M., Kosta, A., Guerlesquin, F., Mignot, T., McBride, M., Cascales, E., Doan, T.. 2021-10-19. Dynamic proton-dependent motors power Type IX secretion and gliding adhesin movement in Flavobacterium. https://doi.org/10.1101/2021.10.19.464928

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