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bioRxiv · 10.1101/2021.06.28.450159

Multiple levels of triggered factors and positive effect of cell-to-cell bottleneck in mutation repair of RNA viruses

Abstract

Debilitating mutation in RNA viruses can be repaired via different mechanisms, while triggering factors of mutation repair were poorly understood. In this study, multiple levels of triggering factors of mutation repair was identified based on genetic damage of tRNA-like structure (TLS) in cucumber mosaic virus (CMV). TLS mutation in different RNAs of CMV distinctively impacted the pathogenicity and mutation repair. Relative quantity defect of RNA2 or quality defect of RNA3 resulting from TLS mutation was correlated with high rate of mutation repair, and TLS mutation of RNA1 failed to be repaired. However, TLS mutation of RNA1 can be repaired in the mixed inoculation with RNA2 having pre-termination mutation of 2b or at the low dose of original inoculation, especially around dilution end-point. Taken together, TLS mutation resulting into quality or quantity defect of viral genome or TLS mutation at low dose around dilution end-point was inclined to be repaired. In addition, different levels of mutation repair of TLS necessarily required the cell-to-cell movement, which implied the positive effect of cell-to-cell bottleneck on evolution of low-fitness virus, a phenomenon opposite to the Muller ratchet. This study provided important revelations on virus evolution and application of viral mild vaccine. Author summaryDue to the low-fidelity of replicase, debilitating RNA viruses can be repaired through different mechanisms, which implied the resilience of RNA viruses. In this study, we identified multiple levels of triggered factors and occurrence occasion of mutation repair using the divided genome of CMV, which contained conserved cis-element tRNA-like structure (TLS) at the 3end. TLS mutation of different RNA in CMV presented different rate of mutation repair from 0-80%. TLS mutation resulting into genomic quality or quantity defect or at low dose around dilution end-point was inclined to be repaired. However, all above types of mutation repair necessarily required cell-to-cell movement, which presented the positive effect of cell-to-cell bottleneck on virus evolution and increased fitness of low-fitness RNA viruses. It is an opposite phenomenon to the Muller ratchet, in which bottleneck always decreased the fitness of viruses. Except to identify the triggering factors of mutation repair in RNA viruses, this study also provided important revelations on creation and application of mild vaccine based on RNA viruses.

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Liu, S., Yu, C., Geng, G., Su, C., Yuan, X.. 2021-06-28. Multiple levels of triggered factors and positive effect of cell-to-cell bottleneck in mutation repair of RNA viruses. https://doi.org/10.1101/2021.06.28.450159

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