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bioRxiv · 10.1101/2021.06.05.447193

Precise annotation of Drosophila mitochondrial genomes leads to insights into AT-rich regions

Liang, G.·Chang, J.·Yau, T. O.·Li, X.·He, B.·Ruan, J.·Bu, W.·Shan, G.
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Abstract

In the present study, we performed precise annotation of Drosophila melanogaster, D. simulans, D. grimshawi, Bactrocera oleae mitochondrial (mt) genomes by pan RNA-seq analysis. Our new annotations corrected or modified some of the previous annotations and two important findings were reported for the first time, including the discovery of the conserved polyA(+) and polyA(-) motifs in the control regions (CRs) of insect mt genomes and the adding of CCAs to the 3 ends of two antisense tRNAs in D. melanogaster mt genome. Using PacBio cDNA-seq data from D. simulans, we precisely annotated the Transcription Initiation Sites (TISs) of the mt Heavy and Light strands in Drosophila mt genomes and reported that the polyA(+) and polyA(-) motifs in the CRs are associated with TISs. The discovery of the conserved polyA(+) and polyA(-) motifs provides insights into many polyA and polyT sequences in CRs of insect mt genomes, leading to reveal the mt transcription and its regulation in invertebrates. In addition, we provided a high-quality, well-curated and precisely annotated D. simulans mt genome (GenBank: MN611461), which should be included into the NCBI RefSeq database to replace the current reference genome NC_005781.

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Liang, G., Chang, J., Yau, T. O., Li, X., He, B., Ruan, J., Bu, W., Shan, G.. 2021-06-07. Precise annotation of Drosophila mitochondrial genomes leads to insights into AT-rich regions. https://doi.org/10.1101/2021.06.05.447193

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