Viability of MS2 and Phi6 Bacteriophages on Carpet and Dust

Nastasi, N.; Renninger, N.; Bope, A.; Cochran, S. J.; Greaves, J.; Haines, S. R.; Balasubrahmaniam, N.; Stuart, K.; Panescu, J.; Bibby, K.; Hull, N. M.; Dannemiller, K. C.

doi:10.1101/2021.05.17.444479

bioRxiv · 10.1101/2021.05.17.444479

Viability of MS2 and Phi6 Bacteriophages on Carpet and Dust

Nastasi, N.·Renninger, N.·Bope, A.·Cochran, S. J.·Greaves, J.·Haines, S. R.·Balasubrahmaniam, N.·Stuart, K.·Panescu, J.·Bibby, K.·Hull, N. M.·Dannemiller, K. C.

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Abstract

Respiratory viral illnesses are commonly spread in the indoor environment through multiple transmission routes, including droplets, aerosols, and direct/indirect contact. Indoors, resuspension of dust from flooring is a major source of human exposure. However, it is critical to determine viral persistence on dust and flooring to better characterize human exposure. The goal of this work is to determine viral viability on two carpet types (cut and looped) and house dust over time and after four different cleaning methods. MS2 and Phi6 bacteriophages were used to represent non-enveloped and enveloped viruses, respectively. These viral surrogates were placed in an artificial saliva solution and nebulized onto carpet or dust. Viability was measured at various time points (0, 1, 2, 3, 4, 24, and 48 hours) and after cleaning (vacuuming, hot water extraction with stain remover, steam, and a disinfection spray). Viability decay was modeled as first-order. MS2 bacteriophages showed slower viability decay rates in dust (-0.11 hr-1), cut carpet (-0.20 hr-1), and looped carpet (-0.09 hr-1) compared to Phi6 (-3.36 hr-1, -1.57 hr-1, and - 0.20 hr-1 respectively). The difference between phages was statistically significant in dust and cut carpet (p<0.05). Viral RNA demonstrated minimal degradation that in most cases was not statistically different from zero over the 48 hours measured (p>0.05). Viable viral concentrations were reduced to below the detection limit for steam and disinfection for both MS2 and Phi6 (p<0.05), while vacuuming and hot water extraction with stain remover showed no significant changes in concentration from uncleaned carpet (p>0.05). This study used viral surrogates and did not model risk of viral transmission via dust. Overall, these results demonstrate that MS2 and Phi6 bacteriophages can remain viable in carpet and dust for several hours to days, and cleaning techniques with heat and disinfectants may be more effective than standard vacuuming for viral removal. Future work should model risk from exposure via dust and flooring for various viruses such as influenza, SARS-CoV-2, and RSV. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=99 SRC="FIGDIR/small/444479v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@1cc0ad9org.highwire.dtl.DTLVardef@dc440corg.highwire.dtl.DTLVardef@f787dcorg.highwire.dtl.DTLVardef@f8bda3_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Nastasi, N., Renninger, N., Bope, A., Cochran, S. J., Greaves, J., Haines, S. R., Balasubrahmaniam, N., Stuart, K., Panescu, J., Bibby, K., Hull, N. M., Dannemiller, K. C.. 2021-05-18. Viability of MS2 and Phi6 Bacteriophages on Carpet and Dust. https://doi.org/10.1101/2021.05.17.444479

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