Nonclassical monocytes are prone to migrate into tumor in diffuse large B-cell lymphoma

Le Gallou, S.; Lhomme, F.; Irish, J. M.; Mingam, A.; Pangault, C.; Monvoisin, C.; Ferrant, J.; Azzaoui, I.; Rossille, D.; Bouabdallah, K.; Damaj, G.; Cartron, G.; Godemer, P.; Le Gouill, S.; Casanovas, O.; Molina, T.; Houot, R.; Lamy, T.; Tarte, K.; Fest, T.; Roussel, M.

doi:10.1101/2021.04.10.439292

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bioRxiv · 10.1101/2021.04.10.439292

Nonclassical monocytes are prone to migrate into tumor in diffuse large B-cell lymphoma

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Abstract

Absolute count of circulating monocytes has been proposed as an independent prognostic factor in diffuse large B-cell lymphoma (DLBCL). However, monocyte nomenclature includes various subsets with pro-, anti-inflammatory, or suppressive functions, and their clinical relevance in DLBCL has been poorly explored. Herein, we broadly assessed circulating monocyte heterogeneity in 91 DLBCL patients. Classical- (cMO, CD14pos CD16neg) and intermediate- (iMO, CD14pos CD16pos) monocytes accumulated in DLBCL peripheral blood and exhibited an inflammatory phenotype. On the opposite, nonclassical monocytes (ncMO, CD14low CD16pos) were decreased in peripheral blood. Tumor-conditioned monocytes presented similarities with ncMO phenotype from DLBCL and were prone to migrate in response to CCL3, CCL5, and CXCL12, and presented similarities with DLBCL-infiltrated myeloid cells, as defined by mass cytometry. Finally, we demonstrated the adverse value of an accumulation of nonclassical monocytes in 2 independent cohorts of DLBCL. Key pointsO_LINonclassical monocytes are prone to migrate to DLBCL tumor C_LIO_LIHigh count of circulating nonclassical monocytes is an independent adverse event in DLBCL C_LI

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Le Gallou, S., Lhomme, F., Irish, J. M., Mingam, A., Pangault, C., Monvoisin, C., Ferrant, J., Azzaoui, I., Rossille, D., Bouabdallah, K., Damaj, G., Cartron, G., Godemer, P., Le Gouill, S., Casanovas, O., Molina, T., Houot, R., Lamy, T., Tarte, K., Fest, T., Roussel, M.. 2021-04-11. Nonclassical monocytes are prone to migrate into tumor in diffuse large B-cell lymphoma. https://doi.org/10.1101/2021.04.10.439292

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