Rapid fall in circulating non-classical monocytes in ST elevation myocardial infarction patients correlates with cardiac injury

Abstract

ObjectiveMyocardial infarction leads to a rapid innate immune response that is ultimately required for repair of damaged heart tissue. We therefore examined circulating monocyte dynamics immediately after reperfusion of the culprit coronary vessel in STEMI patients to determine whether this correlated with level of cardiac injury. A mouse model of cardiac ischaemia/reperfusion injury was subsequently used to establish the degree of monocyte margination to the coronary vasculature that could potentially contribute to the drop in circulating monocytes. Approach and ResultsWe retrospectively analysed blood samples from 51 STEMI patients to assess the number of non-classical (NC), classical and intermediate monocytes immediately following primary percutaneous coronary intervention. Classical and intermediate monocytes showed minimal change. On the other hand circulating numbers of NC monocytes fell by approximately 50% at 90 minutes post-reperfusion. This rapid decrease in NC monocytes was greatest in patients with the largest infarct size (p<0.05) and correlated inversely with left ventricular function (r=0.41, p=0.04). The early fall in NC monocytes post reperfusion was confirmed in a second prospective study of 13 STEMI patients. Furthermore, in a mouse cardiac ischaemia model, there was significant monocyte adhesion to coronary vessel endothelium at 2 hours post-reperfusion pointing to a specific and rapid vessel margination response to cardiac injury. ConclusionsRapid depletion of NC monocytes from the circulation in STEMI patients following coronary artery reperfusion correlates with the level of acute cardiac injury and involves rapid margination to the coronary vasculature. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=130 SRC="FIGDIR/small/428825v1_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@11f758eorg.highwire.dtl.DTLVardef@5eed65org.highwire.dtl.DTLVardef@33a0d9org.highwire.dtl.DTLVardef@1854643_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LI3-5 bullet points that summarize the major findings of the study. C_LI O_LICirculating non classical monocytes show a rapid fall in STEMI patients within 90 minutes of re-opening the culprit coronary artery. C_LIO_LIThe extent of the drop in non classical monocytes correlates with loss of cardiac function and increased infarct size. C_LIO_LIA mouse model of cardiac ischaemia and reperfusion shows rapid margination of monocytes to the coronary vasculature C_LI