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bioRxiv · 10.1101/2020.11.17.377432

An assessment of efficacy of Iodine complex (Renessans) against SARS-CoV-2 in non-human primates (Rhesus macaque)

Nawaz, M.·Ali, M. A.·Ashraf, M. A.·Shabbir, M. Z.·Shabbir, M. A. B.·Altaf, I.·Raza, S.·Rafique, S.·Hassan, S.·Sardar, N.·Mehmood, A.·Aziz, M. W.·Fazal, S.·Khan, M. T.·Atique, M. M.·Asif, A.·Ullah, Z.·Iqbal, M.·Imtiaz, T.·Anwar, M.·Mukhtar, N.·Yaqub, T.
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Abstract

Renessans is an iodine complex which has proven in vitro antiviral activity including Anti-SARS-CoV-2 activity. The present study was designed to determine its efficacy against SARS-CoV-2 in monkeys (Rhesus macaque). A total of 14 monkeys were divided into four groups: A) Prophylactic group (n=03), (B) Treatment group (n=03), (C) infection control group (n=04) and (D) negative control group (n=04) and were housed in BSL-3 Animal facility while group D was housed at another animal house. Group A was administered with Renessans @ 2.85 mg/7 kg from 5 days prior to the infection to 08 days post infections (DPI). Group B was administered with Renessans from 03-08 DPI @ 2.85 mg/7 kg. Group C was administered with WIF only. The infection @ 2 x 106 TCID of SARS-CoV-2 was given to all group monkeys through intranasal and oral route under anesthesia. Nasal swab samples (at different times) and fecal matter on daily basis were collected for the detection of SARS-CoV-2 through real-time quantitative PCR. Three monkeys (one from each of group A, B and C) were euthanized at 07 DPI to determine the gross pathological lesions and SARS-CoV-2 detection from internal tissues. Nasal swabs from all the monkeys from group A, B and C were positive for SARS-CoV-2 at 02 and 07 DPI (Day 05 of treatment). At 14 DPI, all (100%) nasal swabs from group A were negative for SARS-CoV-2 while 50% and 100% were positive from group B and C, respectively. At 21 DPI, monkeys from group B were negative and all in group C were still positive for SARS-CoV-2. Similarly, fecal matter of monkeys in group A and B was returned negative in significantly lesser time as compared to monkeys from infection control group. Based on these research findings it is concluded that the Renessans has in-vivo SARS-CoV-2 activity and may result in early clearance of SARS-CoV-2. Therefore, a clinical trial of the drug in COVID-19 patients may reveal its anti-COVID-19 potential.

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Nawaz, M., Ali, M. A., Ashraf, M. A., Shabbir, M. Z., Shabbir, M. A. B., Altaf, I., Raza, S., Rafique, S., Hassan, S., Sardar, N., Mehmood, A., Aziz, M. W., Fazal, S., Khan, M. T., Atique, M. M., Asif, A., Ullah, Z., Iqbal, M., Imtiaz, T., Anwar, M., Mukhtar, N., Yaqub, T.. 2020-11-18. An assessment of efficacy of Iodine complex (Renessans) against SARS-CoV-2 in non-human primates (Rhesus macaque). https://doi.org/10.1101/2020.11.17.377432

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