Common Marmoset Gut Microbiome Profiles in Health and Intestinal Disease

Sheh, A.; Artim, S. C.; Burns, M. A.; Molina-Mora, J. A.; Lee, M. A.; Dzink-Fox, J.; Muthupalani, S.; Fox, J. G.

doi:10.1101/2020.08.27.268524

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bioRxiv · 10.1101/2020.08.27.268524

Common Marmoset Gut Microbiome Profiles in Health and Intestinal Disease

Sheh, A.·Artim, S. C.·Burns, M. A.·Molina-Mora, J. A.·Lee, M. A.·Dzink-Fox, J.·Muthupalani, S.·Fox, J. G.

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Abstract

Chronic gastrointestinal (GI) diseases are the most common diseases in captive marmosets. The gut microbiome of healthy (n=91), inflammatory bowel disease (IBD) (n=59), and duodenal ulcer/stricture (n=23) captive marmosets was characterized. Healthy marmosets exhibited a "humanized," Bacteroidetes-dominant microbiome. Despite standardized conditions, cohorts subdivided into Prevotella- and Bacteroides-dominant groups based on marmoset source. IBD was highest in a Prevotella-dominant cohort while strictures were highest in a Bacteroides-dominant cohort. Stricture-associated dysbiosis was characterized by Anaerobiospirillum loss and Clostridium perfringens increases. Stricture tissue presented upregulation of lipid metabolism genes and increased abundance of C. perfringens, a causative agent of GI diseases and intestinal strictures in humans. IBD was associated with a lower Bacteroides:P. copri ratio within each source. Consistent with Prevotella-linked diseases, pro-inflammatory genes were upregulated. This report highlights the humanization of the captive marmoset microbiome and its potential as a "humanized" animal model of C. perfringens-induced enteritis/strictures and P. copri-associated IBD.

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Sheh, A., Artim, S. C., Burns, M. A., Molina-Mora, J. A., Lee, M. A., Dzink-Fox, J., Muthupalani, S., Fox, J. G.. 2020-08-27. Common Marmoset Gut Microbiome Profiles in Health and Intestinal Disease. https://doi.org/10.1101/2020.08.27.268524

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