Early postmortem mapping of SARS-CoV-2 RNA in patients with COVID-19 and correlation to tissue damage

Deinhardt-Emmer, S.; Wittschieber, D.; Sanft, J.; Kleemann, S.; Elschner, S.; Haupt, K. F.; Vau, V.; Häring, C.; Rödel, J.; Henke, A.; Ehrhardt, C.; Bauer, M.; Philipp, M.; Gassler, N.; Nietzsche, S.; Löffler, B.; Mall, G.

doi:10.1101/2020.07.01.182550

bioRxiv · 10.1101/2020.07.01.182550

Early postmortem mapping of SARS-CoV-2 RNA in patients with COVID-19 and correlation to tissue damage

Deinhardt-Emmer, S.·Wittschieber, D.·Sanft, J.·Kleemann, S.·Elschner, S.·Haupt, K. F.·Vau, V.·Häring, C.·Rödel, J.·Henke, A.·Ehrhardt, C.·Bauer, M.·Philipp, M.·Gassler, N.·Nietzsche, S.·Löffler, B.·Mall, G.

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Abstract

Clinical observations indicate that COVID-19 is a systemic disease. An investigation of the viral distribution within the human body in correlation to tissue damage can help understanding the pathophysiology of SARS-CoV-2 infection.We present a detailed mapping of viral RNA in 61 tissues and organs of 11 deceased patients with the diagnosis COVID-19. The autopsies were performed within the (very) early postmortem interval (mean: 5.6 hours) to avoid bias due to viral RNA and tissue degradation. Viral loads, blood levels of cytokines, prothrombotic factors as well as macro- and micro-morphology were correlated.Very high (> 104 copies/ml) viral loads were detected in the lungs of most patients and then correlated to severe tissue damage. Intact viral particles could be verified in the lung tissue by transmission electron microscopy. Viral loads in the lymph nodes were associated with a loss of follicular architecture. Viral RNA was detected throughout further extra-pulmonary tissues and organs without visible tissue damage. Inflammatory cytokines as well as the prothrombotic factors were elevated in all patients.In conclusion, the dissemination of SARS-CoV-2-RNA throughout the body supports the hypothesis of a maladaptive host response with viremia and multi-organ dysfunction.View Full Text

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Deinhardt-Emmer, S., Wittschieber, D., Sanft, J., Kleemann, S., Elschner, S., Haupt, K. F., Vau, V., Häring, C., Rödel, J., Henke, A., Ehrhardt, C., Bauer, M., Philipp, M., Gassler, N., Nietzsche, S., Löffler, B., Mall, G.. 2020-07-02. Early postmortem mapping of SARS-CoV-2 RNA in patients with COVID-19 and correlation to tissue damage. https://doi.org/10.1101/2020.07.01.182550

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